Human NOV/CCN3 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3
    Thr32-Met357
    Accession # P48745
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NOV/CCN3 (Catalog # 1640-NV)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Human NOV/CCN3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human NOV/CCN3 Antibody (Catalog # AF1640)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human NOV/CCN3 Biotinylated Antibody (Catalog # BAF1640)
  • ELISA Standard
     
    Recombinant Human NOV/CCN3 Protein, CF (Catalog # 1640-NV)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
NOV/CCN3 in Human Kidney Cancer Tissue. NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1-4). NOV/CCN3 contains an N‑terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38-50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1-4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C‑terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and  alpha 5 beta 1 mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

  • References:
    1. Perbal, B. (2004) Lancet 363:62.
    2. Perbal, B. (2006) Cell Commun. Signal. 4:3.
    3. Martinerie, C. et al. (1994) Oncogene 9:2729.
    4. Snaith, M.R. et al. (1996) Genomics 38:425.
    5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
    6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
    7. Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
    8. Li, C.L. et al. (2002) Mol. Pathol. 55:250.
    9. Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
    10. Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
    11. Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
    12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.
  • Long Name:
    Nephroblastoma Overexpressed Gene
  • Entrez Gene IDs:
    4856 (Human); 18133 (Mouse)
  • Alternate Names:
    CCN family member 3; CCN3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; protein NOV homolog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney
    PLoS ONE, 2016;11(12):e0168240.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
    Authors: Sin WC, Tse M, Planque N, Perbal B, Lampe PD, Naus CC
    J. Biol. Chem., 2009;284(43):29935-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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