Human NrCAM Antibody

Catalog # Availability Size / Price Qty
MAB2034
MAB2034-SP
Product Details
Citations (1)
FAQs
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Reviews

Human NrCAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human NrCAM in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 297906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NrCAM isoform 3
Leu30-Asn600 (Ala526Pro)
Accession # Q14CA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NrCAM

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4 - 5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3 - 5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

References
  1. Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1.
  2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
  3. Mauro, V.P. et al. (1992) 119:191.
  4. Fustig, M. et al. (1999) Dev. Bio. 209:340.
  5. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
  6. Custer, A.W. et al. (2003) J. Neuroscience 23:10032.
Long Name
Neuronal Cell Adhesion Molecule
Entrez Gene IDs
4897 (Human); 319504 (Mouse)
Alternate Names
Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM

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Citation for Human NrCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Global genomic and proteomic analysis identifies biological pathways related to high-risk neuroblastoma.
    Authors: Chen QR, Song YK, Yu LR
    J. Proteome Res., 2010;9(1):373-82.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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