Human Nrf2 Antibody

Catalog # Availability Size / Price Qty
MAB3925
MAB3925-SP
Human Nrf2 Antibody in Data
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (4)

Human Nrf2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 383727
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Nrf2 antibody by Western Blot. View Larger

Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Long Name
NF-E2-related Factor 2
Entrez Gene IDs
4780 (Human); 18024 (Mouse); 83619 (Rat)
Alternate Names
HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2

Product Datasheets

Citations for Human Nrf2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??
    Authors: B Liu, L Xu, X Yu, W Li, X Sun, S Xiao, M Guo, H Wang
    Mol Med Rep, 2018;18(2):1987-1994.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
    Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
    Molecular Medicine Reports, 2018;0(0):1791-3004.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  3. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis
    Authors: G Kontostath, J Zoidakis, M Makridakis, V Lygirou, G Mermelekas, T Papadopoul, K Vougas, A Vlamis-Gar, P Drakakis, D Loutradis, A Vlahou, NP Anagnou, KI Pappa
    Biomed Res Int, 2017;2017(0):4180703.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
    J. Invest. Dermatol., 2012;132(11):2601-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
    Authors: Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL
    J. Immunol., 2011;187(10):5015-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Secondary Antibodies

Reviews for Human Nrf2 Antibody

Average Rating: 3.3 (Based on 4 Reviews)

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Human Nrf2 Antibody
By Anonymous on 12/21/2017
Application: WB Sample Tested: Primary hippocampal neurons Species: Mouse,Rat

***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***


Human/Mouse/Rat Nrf2 Antibody
By Anonymous on 12/10/2017
Application: ICC/IF Sample Tested: Kidney cancer tissue Species: Human

Human Nrf2 Antibody
By Anonymous on 02/06/2017
Application: WB Sample Tested: FL5-12 Species: Mouse

***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***


Human Nrf2 Antibody
By Anonymous on 04/25/2016
Application: WB Sample Tested: mc-1 cell line Species: Rat

RMC-1 cells, rat Muller cells, were cultured and treated, collect with NP-40 buffer, run WB. The problem is the molecular weight, we only see two specific bands around 75 KD marker, we didn't see any band around 120Kd as datasheet from RnD even longer exposure time. We would like to know why. I would like to provide more detailed information if you want to know. Thanks a lot.

***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***