NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot, Neutralization, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 383727
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Product Specifications
Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Met17-Asn605
Accession # Q16236
Specificity
Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Nrf2 Antibody
Detection of Human Nrf2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Nrf2 by Western Blot
Confirmation of the IPA-predicted NRF2 activation in cancer cell lines by Western blot. Western blot analysis with NRF2-specific antibody in four different cell extracts per cell line (SiHa, HeLa, C33A, and HCK1T), corresponding to four biological replicates. Twenty μg was loaded. (a) A protein band of 70 kDa corresponding to NRF2 is detected. Immunoblotting for alpha -tubulin (50 kDa) was applied to ensure the comparable loading of proteins in each lane. Fold expression of NRF2 was assessed relative to HCK1T. (b) The mean NRF2 values for SiHa, HeLa, and C33A were 1.4 ± 0.1 (p < 0.05), 1.1 ± 0.2 (p > 0.05), and 2.3 ± 0.3 (p < 0.05) compared to HCK1T, respectively. Representative images of two biological replicates are shown for each cell line. Graphical representation of densitometry analysis of the results (mean ± SD) is also shown (∗p < 0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28261610), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Nrf2 by Western Blot
Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha ). The mean integrated optical density is related to actin for Nrf2 (B), p53 (C) and mature 8-oxoguanine glycosylase alpha (D) levels. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05, **p < 0.01, ***p < 0.001 as compared to ST unless otherwise stated (horizontal line) for ANOVA with a Fisher post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26892894), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Nrf2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG132 (Catalog # 1748) and siRNA
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG132 (Catalog # 1748) and siRNA
Reviewed Applications
Read 9 reviews rated 3.9 using MAB3925 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Nrf2
Long Name
NF-E2-related Factor 2
Alternate Names
NFE2L2
Gene Symbol
NFE2L2
UniProt
Additional Nrf2 Products
Product Documents for Human Nrf2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Nrf2 Antibody
For research use only
Citations for Human Nrf2 Antibody
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Customer Reviews for Human Nrf2 Antibody (9)
3.9 out of 5
9 Customer Ratings
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Application: Western BlotSample Tested: SK-Mel-28 human malignant melanoma cell lineSpecies: HumanVerified Customer | Posted 12/09/2021
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Application: Western BlotSample Tested: Kidney tissueSpecies: HumanVerified Customer | Posted 09/14/2021
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Application: Western BlotSample Tested: Primary hippocampal neuronsSpecies: Mouse and RatVerified Customer | Posted 12/21/2017
Bio-Techne ResponseIn recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Kidney cancer tissueSpecies: HumanVerified Customer | Posted 12/10/2017
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Application: Western BlotSample Tested: Brain (substantia nigra)Species: MouseVerified Customer | Posted 09/29/2017
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Application: Western BlotSample Tested: FL5-12Species: MouseVerified Customer | Posted 02/06/2017
Bio-Techne ResponseIn recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.
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Application: Western BlotSample Tested: mc-1 cell lineSpecies: RatVerified Customer | Posted 04/25/2016RMC-1 cells, rat Muller cells, were cultured and treated, collect with NP-40 buffer, run WB. The problem is the molecular weight, we only see two specific bands around 75 KD marker, we didn't see any band around 120Kd as datasheet from RnD even longer exposure time. We would like to know why. I would like to provide more detailed information if you want to know. Thanks a lot.
Bio-Techne ResponseIn recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.
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Application: Western BlotSample Tested: See PMID 19282378Species: MouseVerified Customer | Posted 02/20/2015
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Application: Western BlotSample Tested: See PMID 21976775Species: HumanVerified Customer | Posted 02/20/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
