Human OSM R beta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human OSM R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse OSM R beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 469221
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human OSM R beta
    Glu28-Ser739
    Accession # Q99650
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human OSM R beta (Catalog # 4389-OR) under non-reducing conditions only
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of OSM R beta  in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG
F(ab')2 Secondary Antibody (Catalog # F0101B).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSM R beta

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

  • References:
    1. Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. 
    2. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 
    3. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. 
    4. Gearing, D.P. et al. (1992) Science 255:1434. 
    5. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. 
    6. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.  
    7. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. 
    8. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
    9. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
    10. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
    11. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
    12. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
    13. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
    14. Tanaka, M. et al. (2003) Blood 102:3154.
    15. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.
  • Long Name:
    Oncostatin M Receptor beta
  • Entrez Gene IDs:
    9180 (Human); 18414 (Mouse); 310132 (Rat)
  • Alternate Names:
    OSM R beta; OSMR; OSMRB
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