Human Otx2 Antibody

  (14 citations)
(2 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human Otx2 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Otx2
    Met1-Leu289
    Accession # P32243
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Otx2 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Otx2 in NTera‑2 Human Cell Line. Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Otx2 by Simple WesternTM. Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Otx2
Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  • References:
    1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
  • Long Name:
    Orthodenticle Homolog 2
  • Entrez Gene IDs:
    5015 (Human)
  • Alternate Names:
    homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018;50(4):24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina
    Authors: L Todd, L Suarez, C Quinn, AJ Fischer
    Stem Cells, 2017;0(0):.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  3. Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa
    Authors: PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Fr
  4. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
    Authors: Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y
    Development, 2015;142(3):575-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  6. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2015;244(1):86-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015;6(0):6500.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2014;141(2):377-88.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  9. Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
    Authors: Stott, Simon R, Metzakopian, Emmanoui, Lin, Wei, Kaestner, Klaus H, Hen, Rene, Ang, Siew-Lan
    J Neurosci, 2013;33(18):8022-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  10. Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
    Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ
    Br. J. Cancer, 2011;105(6):854-63.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
    Authors: Di Salvio M, Di Giovannantonio LG, Acampora D
    Nat. Neurosci., 2010;13(12):1481-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
    Authors: Zeng H, Guo M, Martins-Taylor K
    PLoS ONE, 2010;5(7):e11853.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007;25(6):1511-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979 Array AF1979
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Excellent
 ICC/IF Human Anonymous 08/22/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Immunocytochemistry/Immunofluorescence: Human Otx2 Antibody [AF1979].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Undifferentiated neural progenitor cells
Species Human

Other Experimental Details

Other Experimental Details ICC on hPSC derived neurl progenitors
  Very Good
 IHC Mouse Anonymous 06/29/2016
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Summary

Application Immunohistochemistry
Sample Tested Embryonic retina
Species Mouse

Other Experimental Details

Other Experimental Details Otx2 staining on frozen mouse embryonic day 17 eyes sections using AF1979 The sections were blocked using 0.01% Triton-X, 2% donkey serum in 1X PBS 1 hour at room temperature. OTX2 was diluted 1:500 using blocking buffer and incubated overnight at 4°C. The secondary antibody used was donkey ant goat conjugated to Alexa Fluor 488 (1:2000).

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