Home / Otx2 / Human Otx2 Antibody

Human Otx2 Antibody

Catalog #: AF1979
75 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1979
AF1979-SP
Detection of Human Otx2 by Western Blot.
4 Images
Product Details
Citations (75)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Otx2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Otx2 antibody by Western Blot. View Larger

Detection of Human Otx2 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Otx2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Otx2 in NTera‑2 Human Cell Line. Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Otx2 antibody in Mouse Embryo by Immunohistochemistry (IHC-P). View Larger

Otx2 in Mouse Embryo. Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human Otx2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Otx2 by Simple WesternTM. Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
Long Name
Orthodenticle Homolog 2
Entrez Gene IDs
5015 (Human)
Alternate Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

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Citations for Human Otx2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

75 Citations: Showing 1 - 10
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  1. Mutations in BCOR, a co-repressor of CRX/OTX2, are associated with early-onset retinal degeneration
    Authors: M Langouët, C Jolicoeur, A Javed, P Mattar, MD Gearhart, SP Daiger, M Bertelsen, L Tranebjærg, ND Rendtorff, K Grønskov, C Jespersgaa, R Chen, Z Sun, H Li, N Alirezaie, J Majewski, VJ Bardwell, R Sui, RK Koenekoop, M Cayouette
    Science Advances, 2022;8(36):eabh2868.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing
    Authors: P Nouri, A Zimmer, S Brüggemann, R Friedrich, R Kühn, N Prakash
    Cells, 2022;11(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Authors: E Boobalan, AH Thompson, RP Alur, DM McGaughey, L Dong, G Shih, ER Vieta-Ferr, IF Onojafe, VK Kalaskar, G Arno, AJ Lotery, B Guan, C Bender, O Memon, L Brinster, C Soleilhavo, L Panman, TC Badea, A Minella, AJ Lopez, SM Thomasy, A Moshiri, D Blain, RB Hufnagel, T Cogliati, K Bharti, BP Brooks
    Investigative Ophthalmology & Visual Science, 2022;63(12):5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  4. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing
    Authors: J Liu, GC Black, SJ Kimber, PI Sergouniot
    Stem Cell Research, 2022;64(0):102880.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
    Authors: J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022;64(0):102893.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
    Authors: M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022;64(0):102892.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats
    Authors: IC Han, LR Bohrer, KN Gibson-Cor, LA Wiley, A Shrestha, BE Harman, C Jiao, EH Sohn, R Wendland, BN Allen, KS Worthingto, RF Mullins, EM Stone, BA Tucker
    Cell Transplantation, 2022;31(0):9636897221104.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants
    Authors: A Manhas, JWS Jahng, CD Vera, SP Shenoy, JW Knowles, JC Wu
    Stem Cell Research, 2022;61(0):102774.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting
    Authors: HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang
    Stem Cell Research, 2022;61(0):102779.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  12. Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently
    Authors: S Touhami, F Béguier, T Yang, S Augustin, C Roubeix, F Blond, JB Conart, JA Sahel, B Bodaghi, C Delarasse, X Guillonnea, F Sennlaub
    International Journal of Molecular Sciences, 2022;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification
    Authors: D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Embryo
    Applications: IHC
  14. Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development
    Authors: L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck, T Thumberger, J Wittbrodt
    Elife, 2021;10(0):.
    Species: Medaka
    Sample Types: Organoid
    Applications: IHC
  15. mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia
    Authors: S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim
    Elife, 2021;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, V Thaker
    Stem Cell Research, 2021;54(0):102432.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Naive stem cell blastocyst model captures human embryo lineage segregation
    Authors: A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo
    Cell Stem Cell, 2021;28(6):1016-1022.e4.
    Species: Human
    Sample Types: Embryo
    Applications: ICC
  18. Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy
    Authors: L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu
    Stem Cell Research, 2021;53(0):102279.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Trophectoderm mechanics direct epiblast shape upon embryo implantation
    Authors: A Weberling, M Zernicka-G
    Cell Reports, 2021;34(3):108655.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  20. Integrin &beta1 coordinates survival and morphogenesis of the embryonic lineage upon implantation and pluripotency transition
    Authors: MA Molè, A Weberling, R Fässler, A Campbell, S Fishel, M Zernicka-G
    Cell Reports, 2021;34(10):108834.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  21. Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation
    Authors: F Nilsson, P Storm, E Sozzi, D Hidalgo Gi, M Birtele, Y Sharma, M Parmar, A Fiorenzano
    Cells, 2021;10(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker
    Stem Cell Research, 2021;59(0):102635.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs
    Authors: J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle
    Nature Communications, 2021;12(1):7322.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  24. Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
    Authors: N Belbachir, C Lai, JW Rhee, Y Zhuge, MV Perez, K Sallam, JC Wu
    Stem Cell Research, 2021;57(0):102605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants
    Authors: P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund
    Stem Cell Reports, 2021;17(1):159-172.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant
    Authors: C Lee, S Cho, C Lai, S Shenoy, R Vagelos, JC Wu
    Stem Cell Research, 2021;59(0):102638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
    Authors: X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li
    Cell Research, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate
    Authors: H De Belly, A Stubb, A Yanagida, C Labouesse, PH Jones, EK Paluch, KJ Chalut
    Cell Stem Cell, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  29. Homeoprotein OTX1 and OTX2 involvement in rat myenteric neuron adaptation after DNBS-induced colitis
    Authors: M Bistoletti, G Micheloni, N Baranzini, A Bosi, A Conti, V Filpa, C Pirrone, G Millefanti, E Moro, A Grimaldi, R Valli, A Baj, F Crema, C Giaroni, G Porta
    PeerJ, 2020;8(0):e8442.
    Species: Rat
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  30. Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation
    Authors: LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou
    Cell Stem Cell, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in M�ller Glial regenerative potential
    Authors: LS VandenBosc, SG Wohl, MS Wilken, M Hooper, C Finkbeiner, K Cox, L Chipman, TA Reh
    Sci Rep, 2020;10(1):13615.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA
    Authors: PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger
    Stem Cell Res, 2020;48(0):101951.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Basement membrane remodelling regulates mouse embryogenesis
    Authors: C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G
    Nature, 2020;582(7811):253-258.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities
    Authors: T Malankhano, L Suldina, E Grigor'eva, S Medvedev, J Minina, K Morozova, E Kiseleva, S Zakian, A Malakhova
    J Pers Med, 2020;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium
    Authors: F Jacob, SR Pather, WK Huang, F Zhang, SZH Wong, H Zhou, B Cubitt, W Fan, CZ Chen, M Xu, M Pradhan, DY Zhang, W Zheng, AG Bang, H Song, J Carlos de, GL Ming
    Cell Stem Cell, 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  36. Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA
    Authors: PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger
    Stem Cell Res, 2020;48(0):101951.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
    Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
    Cell Stem Cell, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells
    Authors: R Kitajima, R Nakai, T Imamura, T Kameda, D Kozuka, H Hirai, H Ito, H Imai, M Imamura
    Stem Cell Res, 2020;44(0):101749.
    Species: Pan troglodytes (Chimpanzee)
    Sample Types: Whole Cells
    Applications: ICC
  39. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020;31(5):107599.
    Species: Human
    Sample Types:
    Applications: IF
  40. Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations
    Authors: M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo
    Stem Cell Res, 2019;35(0):101385.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene
    Authors: AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral
    Stem Cell Res, 2019;41(0):101595.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
    Authors: C Lu, NE Sanjana
    Stem Cell Res, 2019;41(0):101643.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway
    Authors: SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies
    Front Cell Neurosci, 2019;13(0):527.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
    Authors: MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  45. Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene
    Authors: J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares
    Stem Cell Res, 2019;40(0):101570.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene
    Authors: J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares
    Stem Cell Res, 2019;40(0):101569.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene
    Authors: J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares
    Stem Cell Res, 2019;38(0):101468.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene
    Authors: M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares
    Stem Cell Res, 2019;35(0):101386.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations
    Authors: M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow
    Stem Cell Res, 2019;36(0):101389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  50. Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits
    Authors: N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux
    J. Neurosci., 2019;39(6):1005-1019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  51. Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor
    Authors: Y Chen, J Yang, H Geng, L Li, J Li, B Cheng, X Ma, H Li, L Hou
    Dis Model Mech, 2019;12(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  52. Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation
    Authors: R Diacou, Y Zhao, D Zheng, A Cvekl, W Liu
    Cell Rep, 2018;25(9):2510-2523.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  53. In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells
    Authors: SE Harrison, B Sozen, M Zernicka-G
    Nat Protoc, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  54. Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming
    Authors: AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson
    Cell Rep, 2018;24(8):1977-1985.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  55. Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL
    Authors: AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French
    Stem Cell Res, 2018;31(0):157-160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  57. Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene
    Authors: JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French
    Stem Cell Res, 2018;34(0):101361.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation
    Authors: KE Worley, AS Chin, LQ Wan
    Stem Cells Int, 2018;2018(0):1848605.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  59. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018;50(4):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  60. A new genetically engineered mouse model of choroid plexus carcinoma
    Authors: S El Nagar, F Zindy, C Moens, L Martin, D Plassard, MF Roussel, T Lamonerie, N Billon
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  61. Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice
    Authors: X Tang, L Jiao, M Zheng, Y Yan, Q Nie, T Wu, X Wan, G Zhang, Y Li, S Wu, B Jiang, H Cai, P Xu, J Duan, X Lin
    Neuroscience, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina
    Authors: L Todd, L Suarez, C Quinn, AJ Fischer
    Stem Cells, 2017;0(0):.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  63. Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa
    Authors: PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  64. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
    Authors: Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y
    Development, 2015;142(3):575-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  66. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2015;244(1):86-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  67. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015;6(0):6500.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  68. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2014;141(2):377-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  69. Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
    Authors: Stott, Simon R, Metzakopian, Emmanoui, Lin, Wei, Kaestner, Klaus H, Hen, Rene, Ang, Siew-Lan
    J Neurosci, 2013;33(18):8022-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  70. Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
    Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ
    Br. J. Cancer, 2011;105(6):854-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  71. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
    Authors: Zeng H, Guo M, Martins-Taylor K
    PLoS ONE, 2010;5(7):e11853.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  72. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
    Authors: Di Salvio M, Di Giovannantonio LG, Acampora D
    Nat. Neurosci., 2010;13(12):1481-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  73. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  74. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007;25(6):1511-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  75. Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
    Authors: Baulier E, Garcia Diaz A, Corneo B, Farber D
    Stem Cell Res, 0;33(0):274-277.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Isotype Controls

Normal Goat IgG Control

AB-108-C
351 Citations
18 Reviews
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Donkey Anti-Goat IgG Antibody

AF109
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Donkey Anti-Goat IgG Biotinylated Antibody

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3 Citations
1 Review
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
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Flow,IHC,ICC

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NL001
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Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
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Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
12 Citations
1 Image
Flow

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
3 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
10 Citations
2 Reviews
1 Image
Flow

Goat IgG HRP-conjugated Antibody

HAF109
47 Citations
3 Reviews
2 Images
WB,Simple Western

Goat IgG HRP-conjugated Antibody

HAF017
53 Citations
8 Reviews
1 Image
WB

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
17 Citations
1 Image
WB

Goat IgG VisUCyte HRP Polymer

VC004
5 Citations
1 Image
IHC

Rabbit Anti-Goat IgG (H+L) Antibody, X Absorbed

R-401-C-ABS
WB,ELISA

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
2 Citations
1 Review
WB

Reviews for Human Otx2 Antibody

Average Rating: 4.8 (Based on 6 Reviews)

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Human Otx2 Antibody
By Anonymous on 07/12/2021
Application: ICC/IF Sample Tested: Brain,neuron cells Species: Human
Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 06/29/2021
Application: IHC Sample Tested: Adult brain Species: Human
Immunohistochemistry Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 12/21/2020
Application: IHC Sample Tested: Fetal brain Species: Human
Immunohistochemistry Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Alina Filatova on 10/04/2018
Application: ICC/IF Sample Tested: Embryonic brain Species: Mouse

This antibody works very well at 1:1000 in immunofluorescence staining of mouse paraffin-embedded sections. Epitope retrieval: Citrate buffer.

Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 08/22/2016
Application: ICC/IF Sample Tested: Undifferentiated neural progenitor cells Species: Human

ICC on hPSC derived neurl progenitors

Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Isis Trujillo on 06/29/2016
Application: IHC Sample Tested: Embryonic retina Species: Mouse

Otx2 staining on frozen mouse embryonic day 17 eyes sections using AF1979 The sections were blocked using 0.01% Triton-X, 2% donkey serum in 1X PBS 1 hour at room temperature. OTX2 was diluted 1:500 using blocking buffer and incubated overnight at 4°C. The secondary antibody used was donkey ant goat conjugated to Alexa Fluor 488 (1:2000).