Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Avian - Chicken, Chicken, Fish - Danio rerio (Zebrafish), Fish - Oryzias latipes (Medaka or Japanese Rice Fish), Primate - Callithrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Pan troglodytes (Chimpanzee), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Bioassay, Cell Culture, Differentiation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243

Specificity

Detects human Otx2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Otx2 Antibody

Detection of Human Otx2 antibody by Western Blot.

Detection of Human Otx2 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Otx2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Otx2 in NTera‑2 Human Cell Line.

Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Otx2 antibody in Mouse Embryo by Immunohistochemistry (IHC-P).

Otx2 in Mouse Embryo.

Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Otx2 antibody by Simple WesternTM.

Detection of Human Otx2 by Simple WesternTM.

Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human Otx2 by Immunocytochemistry/Immunofluorescence

Detection of Human Otx2 by Immunocytochemistry/Immunofluorescence

Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using Sendai virus vectors and three clones (#1, #2 and #3) were characterized. RT‐PCR confirms the loss of transgenes in hiPSCs‐SLE (lanes 1, 2 and 3), presence (lane 4) in infected fibroblasts (ipF‐SLE) and absence of Sendai viral transgenes in parental fibroblasts (pF‐SLE) (lane 5). Full‐length gels are presented in File S2. (B) The hiPSCs‐SLE colonies expressed alkaline phosphatase. Scale bar, 500 μm. (C) RT‐qPCR analysis of pluripotency genes OCT4, NANOG, SOX2 and REX1 was performed in fibroblasts and in hiPSCs derived from patient with SLE. All expression values are normalized to GAPDH and relative donor fibroblasts. Data are mean ± SEM and all statistical analysis was made between hiPSCs‐SLE clones and relative fibroblasts by Student's t test showing P‐values ≤ .05 in each comparison. (D) PluriTest assays combines novelty score (blue) on x‐axis and pluripotency score (red) on y‐axis. hiPSCs‐SLE localize in the red cloud suggesting the empirical distribution of pluripotent cells compared to non‐pluripotent blue cloud. (E) Representative images of M‐FISH staining show normal karyotypes of hiPSCs‐SLE clones. (F) Immunofluorescence analysis of pluripotent stem cell markers Nanog (green), Oct4 (red) and co‐staining with DAPI (blue) in hiPSCs‐SLE. Scale bar, 50 μm. (G) Representative images of floating and adherent EBs derived from hiPSCs‐SLE at differentiation day 8 and 18, respectively. Scale bar, 500 μm. (H) RT‐qPCR results confirm the capability of hiPSCs‐SLE to differentiate into all three germ layers. The expression levels of GATA4, HAND1 and PAX6 in EBs are relative to undifferentiated hiPSCs. All expression values are normalized to GAPDH and relative hiPSCs. Data are mean ± SEM and all statistical analysis was made between EBs‐SLE and relative hiPSCs‐SLE clones by Student's t test showing P‐values ≤ .05 in each comparison. (I) Immunostaining of Otx2 (ectoderm marker), Sox17 (endoderm marker), Brachyury (mesoderm marker) and co‐staining with DAPI (blue) in EBs derived from hiPSCs‐SLE. Scale bar, 50 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31536674), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Zebrafish Otx2 by Immunohistochemistry

Detection of Zebrafish Otx2 by Immunohistochemistry

Medaka-derived organoids show onset of retinal differentiation. (d) Optical sections showing expression of HuC/D (amacrine&ganglion cells), Otx2 (bipolar&photoreceptor cells),&Prox1 (horizontal cells) in day 4 organoid. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Chicken Otx2 by Immunohistochemistry

Detection of Chicken Otx2 by Immunohistochemistry

The short-term effects of Notch inhibition on RPC reporters. (d) Quantification of the %age of VSX2, Otx2&double marker-positive cells within the electroporated cell population from cell counting. Sectioned chick retinas electroporated ex vivo at E5 with the co-electroporation control CAG::Nuc beta gal with or without CAG::MAML-DN. The retinas cultured for 20 h&immunostained with VSX2 or Otx2 antibodies. The Shapiro–Wilk normality test was used to confirm the normal distribution. * signifies p < 0.05, *** signifies p < 0.001 with a two-tailed student’s t-test. Each point represents one biological replicate. The columns represent mean,&the error bars represent standard deviation. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34267251), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Chicken Otx2 by Flow Cytometry

Detection of Chicken Otx2 by Flow Cytometry

Targeted expression of MAML-DN to cone/HC restricted RPCs does not alter cone formation. (d) Flow cytometry quantification of the %age of Otx2, Lim1, AP2 alpha, OC1ECR22&ThrbCRM2 reporter-positive cells within the ThrbCRM1 lineage traced cell population. Dissociated chick retinal cells electroporated ex vivo at E5 with the co-electroporation control CAG::TdTomato, ThrbCRM1::PhiC31&its responder plasmid,&with or without CAG::MAML-DN. The retinas cultured for 2 days post-electroporation. The Shapiro–Wilk normality test was used to confirm the normal distribution. Mann–Whitney test was used to test significance in ThrbCRM2 quantification. A two-tailed student’s t-test was used to test significance in the rest of the quantifications. * signifies p < 0.05, ** signifies p < 0.01. Each point represents one biological replicate. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34267251), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Zebrafish Otx2 by Immunohistochemistry

Detection of Zebrafish Otx2 by Immunohistochemistry

Medaka primary embryonic pluripotent cells form optic vesicle-like structures. (f) Maximal projection of day 2 organoids&1 dpf embryo generated from Rx3::H2B-GFP transgenic or wild-type blastulae&stained with neural tissue-specific anti-Sox2 (n = 12/12)&anti-Otx2 (n = 10/10) antibodies, co-stained with anti-Rx2&DAPI nuclear stain. hpa, hours post-aggregation; dpf, days post-fertilization. Scale bars: 100 μm.Overview of complexity&variability of organoid morphology generated within one experiment.Overlay of fluorescence&bright-field images of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm.Examples of organoids generated by aggregation of <1000 cells forming one, two,&three optic vesicle-like structures.Fluorescence images, bright-field images,&their overlay of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Zebrafish Otx2 by Immunohistochemistry

Detection of Zebrafish Otx2 by Immunohistochemistry

Examples of organoids generated by aggregation of <1000 cells forming one, two, and three optic vesicle-like structures.Fluorescence images, bright-field images, and their overlay of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Zebrafish Otx2 by Immunohistochemistry

Detection of Zebrafish Otx2 by Immunohistochemistry

Medaka primary embryonic pluripotent cells form optic vesicle-like structures. (e) Bright-field&fluorescent images of day 2 Rx3::H2B-GFP organoids stained with anti-GFP antibody. Optical sections of an organoid (day 2) (n = 9/10)&an embryo (1 dpf) stained with anti-Rx2&anti-Otx2 antibodies. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Otx2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.)

Simple Western

10 µg/mL
Sample: IMR‑32 human neuroblastoma cell line

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line

Reviewed Applications

Read 11 reviews rated 4.8 using AF1979 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Entrez Gene IDs

5015 (Human)

Gene Symbol

OTX2

UniProt

P32243

Additional Otx2 Products

Product Documents for Human Otx2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Otx2 Antibody

For research use only

Citations for Human Otx2 Antibody

Customer Reviews for Human Otx2 Antibody (11)

4.8 out of 5
11 Customer Ratings
5 Stars
82%
4 Stars
18%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 11 reviews Showing All
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  • Human Otx2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Used at 1 ug/mL
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipocytes
    Species: Human
    Verified Customer | Posted 07/17/2025
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Sébastien Brot
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 09/21/2023
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: neuron cells and Brain
    Species: Human
    Verified Customer | Posted 07/12/2021
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 06/29/2021
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Fetal brain
    Species: Human
    Verified Customer | Posted 12/21/2020
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: brain
    Species: pleurodeles waltl
    Verified Customer | Posted 03/30/2020
    In the image, the green 488 is otx2 staining and the blue is DAPI.
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Alina Filatova
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic brain
    Species: Mouse
    Verified Customer | Posted 10/04/2018
    This antibody works very well at 1:1000 in immunofluorescence staining of mouse paraffin-embedded sections. Epitope retrieval: Citrate buffer.
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Undifferentiated neural progenitor cells
    Species: Human
    Verified Customer | Posted 08/22/2016
    ICC on hPSC derived neurl progenitors
    Human Otx2 Antibody AF1979
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: R1 wild type Mouse embryonic Stem Cells
    Species: Mouse
    Verified Customer | Posted 08/04/2016
    Otx2 in R1 wild type mouse embryonic stem cells
    Human Otx2 Antibody AF1979
  • Human Otx2 Antibody
    Name: Isis Trujillo
    Application: Immunohistochemistry
    Sample Tested: Embryonic retina
    Species: Mouse
    Verified Customer | Posted 06/29/2016
    Otx2 staining on frozen mouse embryonic day 17 eyes sections using AF1979 The sections were blocked using 0.01% Triton-X, 2% donkey serum in 1X PBS 1 hour at room temperature. OTX2 was diluted 1:500 using blocking buffer and incubated overnight at 4°C. The secondary antibody used was donkey ant goat conjugated to Alexa Fluor 488 (1:2000).

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