Human p21/CIP1/CDKN1A Antibody

Catalog # Availability Size / Price Qty
AF1047
AF1047-SP
Detection of Human p21/CIP1/CDKN1A by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (4)

Human p21/CIP1/CDKN1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human p21 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p21
Ser2-Pro164
Accession # P38936
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
1-2 µg/500 µg cell lysate
MCF‑7 human breast cancer cell line, see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p21/CIP1/CDKN1A antibody by Western Blot. View Larger

Detection of Human p21/CIP1/CDKN1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry p21/CIP1/CDKN1A antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

p21/CIP1/CDKN1A in MCF‑7 Human Cell Line. p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry p21/CIP1/CDKN1A antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

p21/CIP1/CDKN1A in Human Breast Cancer Tissue. p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of p21/CIP1/CDKN1A antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of p21/CIP1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 µM camphtothecin for 16 hours, then stained with Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Simple Western Detection of Human p21/CIP1/CDKN1A antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human p21/CIP1/CDKN1A by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 16 hours, loaded at 0.2 mg/mL. A specific band was detected for p21/CIP1/CDKN1A at approximately 30 kDa (as indicated) using 5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p21/CIP1/CDKN1A

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Entrez Gene IDs
1026 (Human); 12575 (Mouse); 114851 (Rat)
Alternate Names
CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1

Product Datasheets

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Citations for Human p21/CIP1/CDKN1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function
    Authors: K Koltowska, KS Okuda, M Gloger, M Rondon-Gal, E Mason, J Xuan, S Dudczig, H Chen, H Arnold, R Skoczylas, NI Bower, S Paterson, AK Lagendijk, GJ Baillie, I Leshchiner, C Simons, KA Smith, W Goessling, JK Heath, RB Pearson, E Sanij, S Schulte-Me, BM Hogan
    Nature Cell Biology, 2021;23(11):1136-1147.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors
    Authors: AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack
    Int J Mol Sci, 2020;21(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.
    Authors: Jiang WQ, Zhong ZH, Nguyen A, Henson JD, Toouli CD, Braithwaite AW, Reddel RR
    J. Cell Biol., 2009;185(5):797-810.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human p21/CIP1/CDKN1A Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human p21/CIP1/CDKN1A Antibody
By Jothi Jayavel on 01/25/2018
Application: WB Sample Tested: Prostate cancer Species: Human

Human p21/CIP1/CDKN1A Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Human p21/CIP1/CDKN1A Antibody
By Anonymous on 11/04/2016
Application: IHC Sample Tested: Adult lung Species: Human

Human p21/CIP1/CDKN1A Antibody
By Preethi Vijayaraj on 10/18/2016
Application: ICC/IF Sample Tested: Adult lung Species: Human