Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p21
Ser2-Pro164
Accession # P38936

Specificity

Detects human p21 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human p21/CIP1/CDKN1A Antibody

Detection of Human p21/CIP1/CDKN1A antibody by Western Blot.

Detection of Human p21/CIP1/CDKN1A by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p21/CIP1/CDKN1A antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

p21/CIP1/CDKN1A in MCF‑7 Human Cell Line.

p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
p21/CIP1/CDKN1A antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

p21/CIP1/CDKN1A in Human Breast Cancer Tissue.

p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of p21/CIP1/CDKN1A antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry.

Detection of p21/CIP1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 µM camphtothecin for 16 hours, then stained with Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Detection of Human p21/CIP1/CDKN1A antibody by Simple WesternTM.

Detection of Human p21/CIP1/CDKN1A by Simple WesternTM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 16 hours, loaded at 0.2 mg/mL. A specific band was detected for p21/CIP1/CDKN1A at approximately 30 kDa (as indicated) using 5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human p21/CIP1/CDKN1A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line treated with Camptothecin

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

1-2 µg/500 µg cell lysate
Sample: MCF‑7 human breast cancer cell line, see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with methanol

Simple Western

5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT)

Western Blot

0.5 µg/mL
Sample: Camptothecin-treated MCF‑7 human breast cancer cell line

Reviewed Applications

Read 4 reviews rated 4.3 using AF1047 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p21/CIP1/CDKN1A

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Entrez Gene IDs

1026 (Human); 12575 (Mouse); 114851 (Rat)

Gene Symbol

CDKN1A

UniProt

P38936

Additional p21/CIP1/CDKN1A Products

Product Documents for Human p21/CIP1/CDKN1A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human p21/CIP1/CDKN1A Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Human p21/CIP1/CDKN1A Antibody

Customer Reviews for Human p21/CIP1/CDKN1A Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human p21/CIP1/CDKN1A Antibody
    Name: Jothi Jayavel
    Application: Western Blot
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human p21/CIP1/CDKN1A Antibody AF1047
  • Human p21/CIP1/CDKN1A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human p21/CIP1/CDKN1A Antibody AF1047
  • Human p21/CIP1/CDKN1A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 11/04/2016
    Human p21/CIP1/CDKN1A Antibody AF1047
  • Human p21/CIP1/CDKN1A Antibody
    Name: Preethi Vijayaraj
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 10/18/2016
    Human p21/CIP1/CDKN1A Antibody AF1047

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Protocols

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