Human p21/CIP1/CDKN1A Antibody Summary
Ser2-Pro164
Accession # P38936
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human p21/CIP1/CDKN1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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p21/CIP1/CDKN1A in MCF‑7 Human Cell Line. p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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p21/CIP1/CDKN1A in Human Breast Cancer Tissue. p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of p21/CIP1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 µM camphtothecin for 16 hours, then stained with Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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Detection of Human p21/CIP1/CDKN1A by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 16 hours, loaded at 0.2 mg/mL. A specific band was detected for p21/CIP1/CDKN1A at approximately 30 kDa (as indicated) using 5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p21/CIP1/CDKN1A
p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.
Product Datasheets
Citations for Human p21/CIP1/CDKN1A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells
Authors: Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang
International Journal of Oncology
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The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function
Authors: K Koltowska, KS Okuda, M Gloger, M Rondon-Gal, E Mason, J Xuan, S Dudczig, H Chen, H Arnold, R Skoczylas, NI Bower, S Paterson, AK Lagendijk, GJ Baillie, I Leshchiner, C Simons, KA Smith, W Goessling, JK Heath, RB Pearson, E Sanij, S Schulte-Me, BM Hogan
Nature Cell Biology, 2021-11-08;23(11):1136-1147.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors
Authors: AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack
Int J Mol Sci, 2020-11-05;21(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.
Authors: Jiang WQ, Zhong ZH, Nguyen A, Henson JD, Toouli CD, Braithwaite AW, Reddel RR
J. Cell Biol., 2009-05-25;185(5):797-810.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A high-content cellular senescence screen identifies candidate tumor suppressors, including EPHA3
Authors: Jenni Lahtela, Laura B. Corson, Annabrita Hemmes, Matthew J. Brauer, Sonja Koopal, James Lee et al.
Cell Cycle
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Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2
Authors: Taylor P Enrico, Wayne Stallaert, Elizaveta T Wick, Peter Ngoi, Xianxi Wang, Seth M Rubin et al.
eLife
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IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism
Authors: Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al.
Angiogenesis
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A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo
Authors: JOR Hernandez, X Wang, M Vazquez-Se, M Lopez-Marf, MF Sobral-Rey, A Moran-Horo, M Sundberg, DO Lopez-Cant, CK Probst, GU Ruiz-Espar, K Giannikou, R Abdi, EP Henske, DJ Kwiatkowsk, M Sahin, DR Lemos
Nature Communications, 2021-11-11;12(1):6496.
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