Human p38 beta /MAPK11 Antibody

Name: Human p38 beta /MAPK11 Antibody
Brand: R&D Systems
SKU: MAB3274
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB3274

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 280071

View all p38 beta/MAPK11 Primary Antibodies »

Product Specifications

Immunogen

Human p38 beta /MAPK11 synthetic peptide
EPPKPPGSLEIEQ
Accession # NP_002742

Specificity

Detects human p38 beta /MAPK11 in direct ELISAs. No cross-reactivity with recombinant human p38 alpha, gamma, or delta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human p38 beta /MAPK11 Antibody

p38 beta /MAPK11 in Jurkat Human Cell Line.

p38 beta /MAPK11 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta /MAPK11 Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human p38 beta /MAPK11 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: p38 beta/MAPK11

The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38 delta, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.