Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.
Human Pancreatic Polypeptide/PP Antibody (548301)
R&D Systems | Catalog # MAB6297
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 548301
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Product Specifications
Immunogen
E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298
Ala30-Leu95
Accession # P01298
Specificity
Detects Pancreatic Polypeptide/PP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pancreatic Polypeptide/PP Antibody (548301)
Detection of Human Pancreatic Polypeptide by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human Pancreatic Polypeptide Monoclonal Antibody (Catalog # MAB6297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pancreatic Polypeptide at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Pancreatic Polypeptide/PP Antibody (548301)
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Pancreatic Polypeptide/PP
Additional Pancreatic Polypeptide/PP Products
Product Documents for Human Pancreatic Polypeptide/PP Antibody (548301)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Pancreatic Polypeptide/PP Antibody (548301)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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