Human PDGF R alpha Antibody

Name: Human PDGF R alpha Antibody
Brand: R&D Systems
SKU: MAB322-500
Rating: 5 (2 reviews)

Catalog #: MAB322
12 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB322-SP
MAB322-100
MAB322-500

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Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.

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Citations (12)

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Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PDGF R beta or recombinant mouse PDGF R alpha is observed.

Source

Monoclonal Mouse IgG₁ Clone # 35248

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human PDGF R alpha (Catalog # 322-PR) under non-reducing conditions only

Immunohistochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS‑1 human fetal skin fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF Ra Monoclonal Antibody (Catalog # MAB322). The ND₅₀ is typically 2-10 µg/mL.

Immunohistochemistry

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PDGF R alpha in Human Breast Cancer Tissue. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF Ra Monoclonal Antibody (Catalog # MAB322) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References

Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.

Long Name

Platelet-derived Growth Factor Receptor alpha

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Alternate Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
Cell Death Dis, 2020;11(5):307.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery
Authors: JM Yang, HS Lee, JH Seo, JH Park, IH Gelman, EH Lo, KW Kim
Sci Rep, 2017;7(0):42723.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
PLoS Pathog., 2017;13(4):e1006281.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
Authors: Prignano F, Campolmi P, Bonan P
Dermatol Ther, 2009;22(0):S8-15.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Authors: Li R, Maminishkis A, Wang FE, Miller SS
Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007;177(3):489-500.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
Authors: Wilson HC, Scolding NJ, Raine CS
J. Neuroimmunol., 2006;176(1):162-73.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
Blood, 2006;107(5):1896-902.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
J. Neurosci., 2006;26(47):12174-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
Authors: Kubler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
Prostate, 2005;63(4):385-94.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
J. Cell. Physiol., 2005;203(1):35-43.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.
Authors: Luyt K, Varadi A, Halfpenny CA, Scolding NJ, Molnar E
Biochem. Biophys. Res. Commun., 2004;319(1):120-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC