Human PDGF R alpha Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PDGF R beta or recombinant mouse PDGF R alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 35248
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human PDGF R alpha (Catalog # 322-PR) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS‑1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the WS‑1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322). The ND50 is typically 2‑10 µg/mL.

Immunohistochemistry

PDGF R alpha in Human Breast Cancer Tissue. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

  • References:
    1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
  • Long Name:
    Platelet-derived Growth Factor Receptor alpha
  • Entrez Gene IDs:
    5156 (Human); 18595 (Mouse)
  • Alternate Names:
    alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
    Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
    PLoS Pathog., 2017;13(4):e1006281.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery
    Authors: JM Yang, HS Lee, JH Seo, JH Park, IH Gelman, EH Lo, KW Kim
    Sci Rep, 2017;7(0):42723.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  3. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
    Authors: Prignano F, Campolmi P, Bonan P
    Dermatol Ther, 2009;22(0):S8-15.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
    Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
    J. Neurosci., 2006;26(47):12174-85.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
    Authors: Wilson HC, Scolding NJ, Raine CS
    J. Neuroimmunol., 2006;176(1):162-73.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  8. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2006;107(5):1896-902.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
    Authors: Kubler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
    Prostate, 2005;63(4):385-94.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.
    Authors: Luyt K, Varadi A, Halfpenny CA, Scolding NJ, Molnar E
    Biochem. Biophys. Res. Commun., 2004;319(1):120-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 11 Citations
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Average Rating: 5 (Based on 1 review)

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ApplicationELISA
Sample TestedPurified Standard
SpeciesHuman

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