Human PEAR1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PEAR1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Tenascin R or recombinant mouse Tenascin C is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 492621
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human PEAR1
    Leu21-Ser754
    Accession # Q5VY43
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human PEAR1 (Catalog # 4527-PR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PEAR1 in Human Peripheral Blood Platelets by Flow Cytometry Human peripheral blood platelets were stained with Mouse Anti-Human PEAR1 Monoclonal Antibody (Catalog # MAB4527) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B) and Integrin  alpha  2b/CD41 Fluorescein-conjugated Monoclonal Antibody. Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PEAR1

Platelet endothelial aggregation receptor 1 (PEAR1) is a 150 kDa type I transmembrane protein and member of the MEGF family of proteins (1). Human PEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20 aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 261 aa cytoplasmic region. The ECD consists of 15 EGF-like repeats that vary in length from 39 to 42 aa and contain a consensus sequence of CX1-2GX2GX2-4CX3CX1-3CX1-2GX1-2CX4GX1CX1CX2GX2GX2C (1). The consensus repeat contains six conserved glycine residues and eight conserved cysteine residues, suggesting four disulfide-bonded cysteine pairs in each EGF repeat (1). Within the ECD, there are also five potential sites for N-linked glycosylation. The cytoplasmic region contains five potential Src homology 3-binding, proline-rich domains (1). Mature human PEAR1 is 84% aa identical to mature mouse PEAR1. PEAR1 is most highly expressed in platelets and endothelial cells (1). Functionally, PEAR1 is a receptor for a yet undetermined ligand that signals upon the formation of platelet‑platelet contacts induced both by platelet aggregations and by platelet centrifugation (1). The signaling enhances and stabilizes platelet thrombi (2). Upon aggregation, the surface-expressed protein is tyrosine-phosphorylated (1). This phosphorylation event is inhibited by the alpha IIb beta 3 antagonist eptifibatide, thus demonstrating that PEAR1 tyrosine phosphorylation is dependent on surface contacts between activated platelets (1). PEAR1 can be phosphorylated in an alpha IIb beta 3 integrin-dependent manner on tyrosine (Tyr925) and serine residues (Ser593 and Ser1029) and, potentially, at Tyr804, Tyr943, and Tyr979 (1). Inherited PEAR1 variations that alter expression or function of the platelet signaling molecule could modify agonist-induced aggregation in native platelets (2). In addition, a genetic variant in PEAR1 could be an important determinant of residual platelet function during aspirin treatment, because the COX1/thromboxane A2 pathway will be strongly inhibited by aspirin, and maximal aggregation will then be dependent on other secondary signaling pathways (2).

  • References:
    1. Nanda, N. et al. (2005) J. Biol. Chem. 280:24680.
    2. Herrera-Galeano, J.E. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1484.
  • Long Name:
    Platelet endothelial aggregation receptor 1
  • Entrez Gene IDs:
    375033 (Human); 73182 (Mouse)
  • Alternate Names:
    Jedi; PEAR1; platelet endothelial aggregation receptor 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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