Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 779119
View all Perilipin-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Perilipin‑3
Met1-Lys434
Accession # O60664

Specificity

Detects human Perilipin‑3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Perilipin-2 or rhPerilipin-5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Perilipin‑3 Antibody

Detection of Human Perilipin-3 antibody by Western Blot.

Detection of Human Perilipin‑3 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB7664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Perilipin‑3 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Perilipin-3

M6PRBP1 (mannose 6-phosphate receptor binding protein), gene name PLIN3 (Perilipin-3), also called TIP47 (tail interacting protein of 47 kDa) or PP17 (placental protein 17), is a 47 kDa intracellular transport protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. The 434 amino acid (aa) human Perilipin‑3, which shares approximately 75% aa sequence identity with mouse and rat Perilipin-3, contains a four-helix bundle thought to interact with membranes, and may be phosphorylated and acetylated. A 251 aa form (isoform A) has an alternate start site at aa 184 of the 434 aa form (isoform B). Perilipin-3 is normally found predominantly in the cytoplasm. It is required for uptake of mannose-6 phosphate receptors into endosomes. In the presence of excess lipids, such as in macrophage-derived foam cells, Perilipin-3 may transfer free fatty acids to lipid droplets, promoting their growth.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Entrez Gene IDs

10226 (Human); 66905 (Mouse); 316130 (Rat)

Gene Symbol

PLIN3

UniProt

O60664 (Human)

Additional Perilipin-3 Products

Product Documents for Human Perilipin‑3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Perilipin‑3 Antibody

For research use only

Related Research Areas

Diabetes
Obesity

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