Human Phospho-c-Jun (S63) Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human c-Jun when phosphorylated at S63 in Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1018Y
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human c-Jun S63 site
    Accession # P05412
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Simple Western
    1:100 dilution
    See below
  • Immunocytochemistry
    1:250-1:500 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-c‑Jun (S63) by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c‑Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
c‑Jun in HeLa Human Cell Line. c‑Junphosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human
Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1:500 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-c‑Jun (S63) by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-c‑Jun (S63) at approximately 49 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: c-Jun
c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.
  • Long Name:
    Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1
  • Entrez Gene IDs:
    3725 (Human)
  • Alternate Names:
    Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog
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