Detection of Human Phospho-FAK (Y925) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (Y925) Monoclonal Antibody (Catalog # MAB44671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Focal adhesion kinase 1 (FAK) is a ubiquitously expressed nonreceptor
protein tyrosine kinase that is concentrated in the focal adhesions that form
between cells growing in the presence of extracellular matrix constituents.
This cellular localization is directed by a "Focal Adhesion
Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus.
FAK plays an important role in migration, cell spreading, differentiation,
cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S
phase transition of the cell cycle. Increased expression and/or activity of FAK
in various tumors has been correlated with enhanced migration and invasiveness
of human tumor cells in addition to promoting increased cell proliferation. Src-dependent
phosphorylation of FAK at Tyr925 regulates the effect of FAK on cell migration
and tumor angiogenesis. Human FAK contains one FERM domain (aa 35-355) and one
kinase domain (aa 415-684). Alternate splicing generates additional isoforms
with N-terminal and C-terminal truncations. Within aa 919-930 human, mouse, and
rat FAK share 100% aa sequence identity.
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