Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 743234
View all FAK Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human FAK Y925 site

Specificity

Detects human FAK in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Phospho-FAK (Y925) Antibody

Detection of Human Phospho-FAK (Y925) antibody by Western Blot.

Detection of Human Phospho-FAK (Y925) by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (Y925) Monoclonal Antibody (Catalog # MAB44671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-FAK (Y925) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed nonreceptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation. Src-dependent phosphorylation of FAK at Tyr925 regulates the effect of FAK on cell migration and tumor angiogenesis. Human FAK contains one FERM domain (aa 35-355) and one kinase domain (aa 415-684). Alternate splicing generates additional isoforms with N-terminal and C-terminal truncations. Within aa 919-930 human, mouse, and rat FAK share 100% aa sequence identity.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

Additional FAK Products

Product Documents for Human Phospho-FAK (Y925) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-FAK (Y925) Antibody

For research use only

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail