Human Phospho-PLC-gamma 2 (Y759) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Phospho-PLC-gamma 2 (Y759) in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 744757
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Phosphopeptide containing the human PLC-gamma 2 Y759 site
    Accession # P16885
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-PLC‑ gamma 2 (Y759) by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes or 10 µg/mL Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC‑ gamma 2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC‑ gamma 2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Phospho-PLC‑ gamma 2 (Y759) in Ramos Human Cell Line. PLC‑ gamma 2 phosphorylated at Y759 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line treated (upper panel) or untreated (lower panel) with pervanadate for 5 minutes using Mouse Anti-Human Phospho-PLC‑ gamma 2 (Y759) Monoclonal Antibody (Catalog # MAB7377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.

  • Long Name:
    Phospholipase C gamma 2
  • Entrez Gene IDs:
    5336 (Human); 234779 (Mouse); 29337 (Rat)
  • Alternate Names:
    EC 3.1.4.11; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phospholipase C-IV; PLCG2; PLCgamma 2; PLC-gamma 2; PLC-gamma-2; PLC-IV
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