Human Phospho-PLC‑ gamma 2 (Y759) Antibody

Name: Human Phospho-PLC‑ gamma 2 (Y759) Antibody
Brand: R&D Systems
SKU: MAB7377
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB7377

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 744757

View all PLC-gamma 2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human PLC-gamma 2 Y759 site
Accession # P16885

Specificity

Detects human Phospho-PLC-gamma 2 (Y759) in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Phospho-PLC‑ gamma 2 (Y759) Antibody

Detection of Human Phospho-PLC‑ gamma 2 (Y759) by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes or 10 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC- gamma2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-PLC‑ gamma 2 (Y759) in Ramos Human Cell Line.

PLC- gamma2 phosphorylated at Y759 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line treated (upper panel) or untreated (lower panel) with pervanadate for 5 minutes using Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Phospho-PLC‑ gamma 2 (Y759) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line treated with pervanadate (PV)

Western Blot

0.2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line treated with Pervanadate (PV) or Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.