Human Phospho-SYK (Y525/Y526) Antibody

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Detection of Human Phospho-SYK (Y525/Y526) by Western Blot.
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Product Details
Citations (2)
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Human Phospho-SYK (Y525/Y526) Antibody Summary

Species Reactivity
Detects human Phospho-SYK when phosphorylated at Y525/Y526 in Western blots.
Monoclonal Mouse IgG2B Clone # 616268
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing human SYK Y525/Y526 sites
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-SYK (Y525/Y526) by Western Blot. View Larger

Detection of Human Phospho-SYK (Y525/Y526) by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 µg/mL IgM for 2 minutes and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 2nM pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Human Phospho-SYK (Y525/Y526) Monoclonal Antibody (Catalog # MAB6459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phopho-SYK (Y525/Y526) at approximately 80 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with mouse anti-SYK (lower panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SYK

Spleen tyrosine kinase (SYK; also tyrosine-protein kinase SYK) is a 70-80 kDa, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP-70 subfamily of proteins. Human SYK is 635 amino acids (aa) in length and contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform that has a deletion of aa 371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of B-cell antigen receptor-stimulated responses.

Long Name
Spleen Tyrosine Kinase
Entrez Gene IDs
6850 (Human); 20963 (Mouse); 25155 (Rat)
Alternate Names
DKFZp313N1010; EC 2.7.10; EC; FLJ25043; spleen tyrosine kinaseFLJ37489; SYK; tyrosine-protein kinase SYK

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Citations for Human Phospho-SYK (Y525/Y526) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SYK is activated by mutated MYD88 and drives pro-survival signaling in MYD88 driven B-cell lymphomas
    Authors: M Munshi, X Liu, JG Chen, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, C Jimenez, GG Chan, ZR Hunter, ML Palomba, KV Argyropoul, K Meid, A Keezer, J Gustine, T Dubeau, JJ Castillo, CJ Patterson, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang
    Blood Cancer J, 2020;10(1):12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling
    Authors: S Hasan, WU Rahman, P Sebo, R Osicka
    Toxins (Basel), 2019;11(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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