|Detection of Human Phospho-SYK (Y525/Y526) by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 µg/mL IgM for 2 minutes and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 2nM pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Human Phospho-SYK (Y525/Y526) Monoclonal Antibody (Catalog # MAB6459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phopho-SYK (Y525/Y526) at approximately 80 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with mouse anti-SYK (lower panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Spleen tyrosine kinase (SYK; also tyrosine-protein kinase SYK) is a 70-80 kDa, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP-70 subfamily of proteins. Human SYK is 635 amino acids (aa) in length and contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform that has a deletion of aa 371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of B-cell antigen receptor-stimulated responses.
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