Human PIEZO1 Antibody (1081418)

R&D Systems | Catalog # MAB11557

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1081418
View all PIEZO1 Primary Antibodies »

Product Specifications

Immunogen

PIEZO1 containing synthetic peptide
Accession # Q92508

Specificity

Detects a synthetic peptide around aa 150 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PIEZO1 Antibody (1081418)

Detection of PIEZO1 in Human Kidney.

PIEZO1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PIEZO1 Monoclonal Antibody (Catalog # MAB11557) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PIEZO1 Antibody (1081418)

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIEZO1

Piezo1 is an 287kDa mechanosensitive nonselective cation channel, extensively distributed in multiple tissues and cell types.  Piezo1 plays an important role in numerus physiological processes, such as determining vascular structure, urine osmoregulation, and blood pressure homeostasis.  In the kidney, Piezo1 expression is found in the renal corpuscle, convoluted tubules, and collecting ducts.

References

  1. Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W. Mechanosensitive Piezo1 channels mediate renal fibrosis. JCI Insight. 2022 Apr 8;7(7):e152330. doi: 10.1172/jci.insight.152330. PMID: 35230979; PMCID: PMC9057604.
  2. Carrisoza-Gaytan R, Kroll KT, Hiratsuka K, Gupta NR, Morizane R, Lewis JA, Satlin LM. Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca2+ signaling. Am J Physiol Cell Physiol. 2023 Mar 1;324(3):C757-C768. doi: 10.1152/ajpcell.00288.2022. Epub 2023 Feb 6. PMID: 36745528; PMCID: PMC10027089.

Long Name

Piezo-type mechanosensitive ion channel component 1

Alternate Names

FAM38A, KIAA0233, Mib, Protein FAM38A

Entrez Gene IDs

9780 (Human); 361430 (Rat)

Gene Symbol

PIEZO1

UniProt

Q92508 (Human)

Additional PIEZO1 Products

Product Documents for Human PIEZO1 Antibody (1081418)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PIEZO1 Antibody (1081418)

For research use only

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Protocols

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