Human Plexin A1 Antibody

Catalog # Availability Size / Price Qty
Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry.
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Product Details
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Human Plexin A1 Antibody Summary

Species Reactivity
Detects human Plexin A1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 708954
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Plexin A1
Accession # Q9UIW2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Plexin A1 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Plexin A1 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Plexin A1 in Human PBMCs. Plexin A1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) differentiated to dendritic cells using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to transmembrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names

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