Human Plexin A1 Antibody

Catalog # Availability Size / Price Qty
MAB65361-100
MAB65361-SP
Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry.
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Human Plexin A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin A1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 708954
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin A1
Tyr964-Leu1152
Accession # Q9UIW2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Plexin A1 in Human PBMCs. View Larger

Plexin A1 in Human PBMCs. Plexin A1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) differentiated to dendritic cells using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to transmembrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names
NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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