Detection of Human PP4 Catalytic Subunit by Western Blot. |
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PP4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP4 Catalytic Subunit at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
The catalytic subunit of Protein Phosphatase 4 (PP4), also called PPX, is a serine/threonine protein phosphatase with about 65% homology to PP2A. Although PP4 has the same heterotrimeric subunit structure as PP2A and can use many of the PP2A regulatory subunits, PP4 also has its own unique scaffolding and regulatory subunits. PP4 is largely found in the centrosome of the nucleus where it may play a role in modulating transcription factor phosphorylation by JNK. In Drosophila, cmm mutants have low levels of PP4 and are unable to complete mitosis due to a deficit in microtubule assembly.
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