Human PPAR gamma /NR1C3 Antibody

R&D Systems | Catalog # PP-A3409A-00

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation, Gel Supershift Assay

Cited:

Proximity Ligation Assay (PLA)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # A3409A
View all PPAR gamma/NR1C3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PPAR gamma/NR1C3
Amino Acids 3-108

Specificity

This antibody specifically recognizes human PPAR gamma 1 and gamma 2 and cross-reacts with mouse and rat PPAR gamma 1 and gamma 2. This antibody does not recognize human PPAR alpha and PPAR delta. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human PPAR gamma /NR1C3 Antibody

Application
Recommended Usage

Gel Supershift Assay

Optimal dilutions should be determined by each laboratory.

Immunohistochemistry

This antibody can be used at 10 μg/mL with the appropriate secondary reagents to detect human PPAR gamma.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human PPAR gamma.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PPAR gamma/NR1C3

Peroxisome Proliferator-Activated Receptor gamma (PPAR gamma ; NR1C3) is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR gamma. PPAR gamma is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPAR gamma plays important roles in lipid and glucose metabolism and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three known family members are called PPAR alpha, delta, and gamma. Three N-terminal isoforms, called gamma 1, gamma 2 and gamma 3, are known to arise by alternative splicing and promoter usage from the PPAR gamma gene. RXR is an obligate partner for PPAR.

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Entrez Gene IDs

5468 (Human)

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for Human PPAR gamma /NR1C3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PPAR gamma /NR1C3 Antibody

For research use only

Citations for Human PPAR gamma /NR1C3 Antibody

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Protocols

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FAQs

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