Human/Primate ST2/IL-33 R Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ST2/IL-33 R in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ST2L, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 RA, rhIL-1 alpha, or rhIL-1 beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 97203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R
    Lys19-Phe328
    Accession # BAA02233
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human ST2/IL‑1 R4 Fc Chimera (Catalog # 523-ST)
    • Human/Primate ST2/IL-33 R Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate ST2/IL-33 R Antibody (Catalog # MAB523)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate ST2/IL-33 R Biotinylated Antibody (Catalog # BAF523)
  • ELISA Standard
     
    Recombinant Human ST2/IL-33 R Fc Chimera Protein, CF (Catalog # 523-ST)
  • Neutralization
    Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC).The Neutralization Dose (ND50) is typically 0.3-1.5 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN-gamma  Secretion Induced by
IL‑33 and Neutralization by Human ST2/IL-33 R Antibody.

Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND50 is typically 0.3-1.5 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST2/IL-33 R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15).

  • References:
    1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
    2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
    3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
    4. Li, H. et al. (2000) Genomics 67:284.
    5. Schmitz, J. et al. (2005) Immunity 23:479.
    6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
    7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
    8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
    9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
    10. Palmer, G. et al. (2008) Cytokine 42:358.
    11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
    12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
    13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
    14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
    15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  • Long Name:
    Interleukin 33 Receptor
  • Entrez Gene IDs:
    9173 (Human); 17082 (Mouse)
  • Alternate Names:
    DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.
    Authors: Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T
    Clin. Exp. Allergy, 2012;42(2):218-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures.
    Authors: Blom L, Poulsen L
    J Immunol, 2012;189(9):4331-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
    Authors: Yasuo T, Kitaya K
    Fertil. Steril., 2009;92(2):709-21.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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