Human Pro- Collagen II Antibody

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Human Pro-Collagen II ELISA Standard Curve.
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Product Details
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Human Pro- Collagen II Antibody Summary

Species Reactivity
Detects human Pro-Collagen II in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 819447
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Pro-Collagen II
Gln26-Arg266 and Gly957-Leu1487
Accession # P02458
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-Collagen II DuoSet ELISA Kit (Catalog # DY7589-05) for convenient development of a sandwich ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human Pro-Collagen II ELISA Standard Curve. Recombinant Pro-Human Collagen II protein was serially diluted 2-fold and captured by Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen II

Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis (1). This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.

  1. Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.
Entrez Gene IDs
1280 (Human); 12824 (Mouse); 25412 (Rat)
Alternate Names
Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; Collagen II; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC

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Isotype Controls

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Secondary Antibodies

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