Human Pro- Collagen II Antibody
Human Pro- Collagen II Antibody Summary
Gln26-Arg266 and Gly957-Leu1487
Accession # P02458
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-Collagen II DuoSet ELISA Kit (Catalog # DY7589-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Pro-Collagen II ELISA Standard Curve. Recombinant Human Pro-Collagen II protein was serially diluted 2-fold and captured by Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen II
Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis (1). This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.
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