Prominin 2 in HepG2 Human Cell Line.
Prominin 2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Prominin 2 Monoclonal Antibody (Catalog # MAB2024) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prominin 2
Prominin 2 is a pentaspan membrane glycoprotein predominantly expressed in neuroepithelial cells, hematopoietic stem cells and epithelial cells of the adult kidney and digestive tract (1, 2). Prominin 2 is a 112-kDa glycoprotein structurally
related to prominin 1 (CD133). The amino acid identity between prominin 1 and prominin 2 is
(<30%), but they exhibit some redundant functions.
Fargeas, C.A. et al. (2003) J. Biol. Chem. 278(10):8586.
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