Detection of Human Prostaglandin I2 Synthase by Western Blot.
Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Prostaglandin I2 Synthase by Simple WesternTM.
Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer, loaded at 0.5 mg/mL. A specific band was detected for Prostaglandin I2 Synthase at approximately 55 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostaglandin I2 Synthase
PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma. Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.
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