Human Prostaglandin I2 Synthase Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Prostaglandin I2 Synthase in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 852307
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Prostaglandin I2 Synthase
    Arg21-Pro500
    Accession # Q16647
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Prostaglandin I2 Synthase by Western Blot. Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Prostaglandin I2 Synthase by Simple WesternTM. Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer, loaded at 0.5 mg/mL. A specific band was detected for Prostaglandin I2 Synthase at approximately 55 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostaglandin I2 Synthase
PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma. Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.
  • Entrez Gene IDs:
    5740 (Human); 19223 (Mouse); 25527 (Rat)
  • Alternate Names:
    CYP8; CYP8A1; CYP8A1PTGI; CYP8prostacyclin synthase; EC 5.3.99.4; MGC126858; MGC126860; PGIS; PGIScytochrome P450, family 8, subfamily A, polypeptide 1; Prostacyclin Synthase; prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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