Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 852307
View all Prostaglandin I2 Synthase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Prostaglandin I2 Synthase
Arg21-Pro500
Accession # Q16647

Specificity

Detects human Prostaglandin I2 Synthase in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Prostaglandin I2 Synthase Antibody

Detection of Human Prostaglandin I2 Synthase antibody by Western Blot.

Detection of Human Prostaglandin I2 Synthase by Western Blot.

Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Prostaglandin I2 Synthase antibody by Simple WesternTM.

Detection of Human Prostaglandin I2 Synthase by Simple WesternTM.

Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer, loaded at 0.5 mg/mL. A specific band was detected for Prostaglandin I2 Synthase at approximately 55 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Prostaglandin I2 Synthase Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer

Western Blot

1 µg/mL
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovary tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prostaglandin I2 Synthase

PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma. Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.

Alternate Names

CYP8, CYP8A1, PGIS, Prostacyclin Synthase, PTGI

Entrez Gene IDs

5740 (Human); 19223 (Mouse); 25527 (Rat)

Gene Symbol

PTGIS

UniProt

Q16647 (Human)

Additional Prostaglandin I2 Synthase Products

Product Documents for Human Prostaglandin I2 Synthase Antibody

Certificate of Analysis

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Product Specific Notices for Human Prostaglandin I2 Synthase Antibody

For research use only

Citations for Human Prostaglandin I2 Synthase Antibody

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