Human Rab13 Antibody

Catalog # Availability Size / Price Qty
MAB8305
MAB8305-SP
Human Rab13 Antibody in Western Blot
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Rab13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Rab13 in direct ELISA and Western Blot.
Source
Monoclonal Mouse IgG2A Clone # 863028
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Rab13
Lys94-Thr191
Accession # P51153
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Rab13 by Western Blot. View Larger

Detection of Human Rab13 by Western Blot. Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Rab13 in Caco‑2 Human Cell Line. View Larger

Rab13 in Caco‑2 Human Cell Line. Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rab13

Ras-related protein Rab13 is a 23 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab13 is detected in endothelial cells and a variety of epithelia, including intestine, lung, kidney and liver.  Rab13 may participate in the assembly and activity of tight junctions, as it colocalizes with the marker ZO-1 in polarized epithelial cells.  In addition, insulin promotes GTP loading of Rab13, potentially regulating GLUT4 translocation in skeletal muscle cells.  Human Rab13 is 203 amino acids (aa) in length, with residues 201-203 removed in its mature form.  Over aa 94-191, human Rab13 shares 89 and 90% identity with mouse and rat Rab13, respectively.

Long Name
RAs Genes from Brain Protein 13
Entrez Gene IDs
5872 (Human); 68328 (Mouse); 81756 (Rat)
Alternate Names
Cell growth-inhibiting gene 4 protein; growth-inhibiting gene 4 protein; Rab13; RAB13, member RAS oncogene family; RAS-associated protein RAB13; ras-related protein Rab-13

Product Datasheets

Citation for Human Rab13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis
    Authors: DR Gulbranson, EM Davis, BA Demmitt, Y Ouyang, Y Ye, H Yu, J Shen
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Rab13 Antibody

There are currently no reviews for this product. Be the first to review Human Rab13 Antibody and earn rewards!

Have you used Human Rab13 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review