Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 863028
View all Rab13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rab13
Lys94-Thr191
Accession # P51153

Specificity

Detects human Rab13 in direct ELISA and Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Rab13 Antibody

Detection of Human Rab13 antibody by Western Blot.

Detection of Human Rab13 by Western Blot.

Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Rab13 antibody in Caco-2 Human Cell Line by Immunocytochemistry (ICC).

Rab13 in Caco‑2 Human Cell Line.

Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Rab13 Specificity Using Knockout Cell Line.

Western Blot Shows Rab13 Specificity Using Knockout Cell Line.

Western blot shows lysates of HEK-293T parental cell line and Rab13 knockout HEK-293T cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental HEK-293T cell line, but is not detectable in knockout HEK-293T cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Western Blot Shows Rab13 Specificity Using Knockdown Cell Line.

Western Blot Shows Rab13 Specificity Using Knockdown Cell Line.

Western blot shows lysates of U87 MG parental cell line and Rab13 knockout U87 MG cell line (KD). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental U87 MG cell line, but is significantly reduced in the U87 MG cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Rab13 Specificity is Shown by Immunocytochemistry in Knockdown Cell Line.

Rab13 Specificity is Shown by Immunocytochemistry in Knockdown Cell Line.

U‑87 MG human glioblastoma/astrocytoma parental cell line WT and Rab13 U-87 MG KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human Rab13 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Caco‑2 human colorectal adenocarcinoma cell line

Knockout Validated

Rab13 is specifically detected in HEK-293T parental cell line but is not detectable in knockout HEK-293T cell line.

Western Blot

2 µg/mL
Sample: Human lung tissue and human aorta tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB8305 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rab13

Ras-related protein Rab13 is a 23 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab13 is detected in endothelial cells and a variety of epithelia, including intestine, lung, kidney and liver.  Rab13 may participate in the assembly and activity of tight junctions, as it colocalizes with the marker ZO-1 in polarized epithelial cells.  In addition, insulin promotes GTP loading of Rab13, potentially regulating GLUT4 translocation in skeletal muscle cells.  Human Rab13 is 203 amino acids (aa) in length, with residues 201-203 removed in its mature form.  Over aa 94-191, human Rab13 shares 89 and 90% identity with mouse and rat Rab13, respectively.

Long Name

RAs Genes from Brain Protein 13

Alternate Names

Cell growth-inhibiting gene 4 protein, growth-inhibiting gene 4 protein, RAB13, member RAS oncogene family, RAS-associated protein RAB13, ras-related protein Rab-13

Entrez Gene IDs

5872 (Human); 68328 (Mouse); 81756 (Rat)

Gene Symbol

RAB13

UniProt

P51153

Additional Rab13 Products

Product Documents for Human Rab13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rab13 Antibody

For research use only

Related Research Areas

Epithelial Cell Polarity

Citations for Human Rab13 Antibody

Customer Reviews for Human Rab13 Antibody (2)

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  • Human Rab13 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Aortic endothelial cells
    Species: Human
    Verified Customer | Posted 07/08/2022
    Human Rab13 Antibody MAB8305
  • Human Rab13 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neuroblasts
    Species: Human
    Verified Customer | Posted 02/17/2022
    Human Rab13 Antibody MAB8305

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