Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # D18
View all Laminin gamma 1 Primary Antibodies »

Product Specifications

Immunogen

Rat glomerular basement membrane

Specificity

Detects human and rat Laminin gamma 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Rat Laminin  gamma 1 Antibody

Laminin ?1 antibody in LS180 Human Cell Line by Immunocytochemistry (ICC).

Laminin gamma 1 in LS180 Human Cell Line.

Laminin gamma1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat Laminin  gamma 1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunoprecipitation

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.

Western Blot

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.

Reviewed Applications

Read 3 reviews rated 3.7 using MAB2139 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin gamma 1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentation, cell shape and migration, and maintenance of tissue phenotypes and survivial (1). Laminin gamma 1, also known as Laminin B2, is the gamma subunit of Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin).

References

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.

Alternate Names

LAMC1, Laminin B2

Entrez Gene IDs

3915 (Human); 117036 (Rat)

Gene Symbol

LAMC1

Additional Laminin gamma 1 Products

Product Documents for Human/Rat Laminin  gamma 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Rat Laminin  gamma 1 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human/Rat Laminin  gamma 1 Antibody

Customer Reviews for Human/Rat Laminin  gamma 1 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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  • Human/Rat Laminin  gamma 1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Rat Laminin  gamma 1 Antibody MAB2139
  • Human/Rat Laminin  gamma 1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/09/2019
  • Human/Rat Laminin  gamma 1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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Protocols

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