Human/Rat Laminin  gamma 1 Antibody

Catalog # Availability Size / Price Qty
MAB2139
MAB2139-SP
Laminin  gamma 1 in LS180 Human Cell Line.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)

Human/Rat Laminin  gamma 1 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat Laminin gamma 1.
Source
Monoclonal Mouse IgG2A Clone # D18
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat glomerular basement membrane
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.

 
Immunoprecipitation
Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Laminin ?1 antibody in LS180 Human Cell Line by Immunocytochemistry (ICC). View Larger

Laminin gamma 1 in LS180 Human Cell Line. Laminin gamma1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin gamma 1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentation, cell shape and migration, and maintenance of tissue phenotypes and survivial (1). Laminin gamma 1, also known as Laminin B2, is the gamma subunit of Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin).

References
  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
Entrez Gene IDs
3915 (Human); 117036 (Rat)
Alternate Names
LAMB2Laminin-7 subunit gamma; LAMC1; Laminin B2 chain; Laminin B2; Laminin gamma 1; laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; MGC87297; S-LAM gamma; S-laminin subunit gamma

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Citations for Human/Rat Laminin  gamma 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PLAC1 is essential for FGF7/FGFRIIIb-induced Akt-mediated cancer cell proliferation
    Authors: DB Roldán, M Grimmler, C Hartmann, S Hubich-Rau, T Bei beta ert, C Paret, G Cagna, C Rohde, S Wöll, M Koslowski, Ö Türeci, U Sahin
    Oncotarget, 2020;11(20):1862-1875.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Authors: Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
    J. Biol. Chem., 1992;267(3):2014-22.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Rat Laminin  gamma 1 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human/Rat Laminin  gamma 1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Rat Laminin  gamma 1 Antibody
By Anonymous on 01/09/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Rat Laminin  gamma 1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human