Laminin gamma 1 in LS180 Human Cell Line.
Laminin gamma 1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma 1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin gamma 1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentation, cell shape and migration, and maintenance of tissue phenotypes and survivial (1). Laminin gamma 1, also known as Laminin B2, is the gamma subunit of Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin).
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