Human/Rat Laminin  gamma 1 Antibody

(1 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat Laminin gamma 1.
  • Source
    Monoclonal Mouse IgG2A Clone # D18
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rat glomerular basement membrane
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.

  • Immunoprecipitation
    Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Laminin  gamma 1 in LS180 Human Cell Line. Laminin  gamma 1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin  gamma 1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin gamma 1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentation, cell shape and migration, and maintenance of tissue phenotypes and survivial (1). Laminin gamma 1, also known as Laminin B2, is the gamma subunit of Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin).
  • References:
    1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  • Entrez Gene IDs:
    3915 (Human); 117036 (Rat)
  • Alternate Names:
    LAMB2Laminin-7 subunit gamma; LAMC1; Laminin B2 chain; Laminin B2; Laminin gamma 1; laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; MGC87297; S-LAM gamma; S-laminin subunit gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Authors: Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
    J. Biol. Chem., 1992;267(3):2014-22.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
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