Human/Rat SOX10 Antibody

(6 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10 (1).
  • Source
    Monoclonal Mouse IgG1 Clone # 20B7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat SOX10
    aa 1-118
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX10
SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.
  • References:
    1. Lo, L. et al. (2002) Development 129:1553.
  • Long Name:
    Transcription Factor SOX10
  • Entrez Gene IDs:
    6663 (Human)
  • Alternate Names:
    DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Oligodendroglial maturation is dependent on intracellular protein shuttling.
    Authors: Gottle P, Sabo J, Heinen A, Venables G, Torres K, Tzekova N, Parras C, Kremer D, Hartung H, Cate H, Kury P
    J Neurosci, 2015;35(3):906-19.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  2. Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.
    Authors: Xu W, Sun Y, Zhang J, Xu K, Pan L, He L, Song Y, Njunge L, Xu Z, Chiang M, Sung K, Chuong C, Yang L
    Stem Cells Dev, 2015;24(7):857-68.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  3. Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system.
    Authors: Coquenlorge S, Duchalais E, Chevalier J, Cossais F, Rolli-Derkinderen M, Neunlist M
    J Neuroinflammation, 2014;11(1):202.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  4. HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
    Authors: Jacob C, Lotscher P, Engler S, Baggiolini A, Varum Tavares S, Brugger V, John N, Buchmann-Moller S, Snider P, Conway S, Yamaguchi T, Matthias P, Sommer L, Mantei N, Suter U
    J Neurosci, 2014;34(17):6112-22.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. HDAC1 and HDAC2 control the transcriptional program of myelination and the survival of Schwann cells.
    Authors: Jacob C, Christen CN, Pereira JA, Somandin C, Baggiolini A, Lotscher P, Ozcelik M, Tricaud N, Meijer D, Yamaguchi T, Matthias P, Suter U
    Nat. Neurosci., 2011;14(4):429-36.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  6. A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
    Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
    Stem Cells, 2007;26(1):163-72.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Isotype Controls
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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