Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 20B7
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat SOX10
aa 1-118

Specificity

Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10 (1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Rat SOX10 Antibody

SOX10 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX10 in BG01V Human Embryonic Stem Cells.

SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX10 antibody in Human Melanoma Tissue by Immunohistochemistry (IHC-P).

SOX10 in Human Melanoma Tissue.

SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Rat SOX10 by Immunocytochemistry/Immunofluorescence

Detection of Rat SOX10 by Immunocytochemistry/Immunofluorescence

Identification of neural crest-derived DPCs. ((a)–(c)) Immunofluorescent cytochemical staining revealed expression of NCSCs-specific markers in DPCs. Primary DPCs were double positive for P75 and Sox10 (a), P75 and Nestin (b), and were positive for Sox9 (c). (d) RT-PCR analysis confirmed the expression of dermal papillae markers ALP and Sox2, and the NCSCs markers Nestin, P75, Sox9, Twist1, and AP2 alpha in DPCs, as well as BMSCs. Scale bar = 20 μm ((a)–(c)). DPCs-P#: DPCs at passage #; BMSCs-P#: BMSCs at passage #. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25045659), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

JCV T-antigen is expressed in neural crest cells.Fluorescent images of bone marrow cells isolated from JCV T-antigen transgenic mice cultured in neural conditions show that these cells are positive for p75 (A,B), nestin (D,E) and SOX-10 (G,H), which are all markers of the neural crest. JCV T-antigen (J,K) was detected in the nucleus of p75 positive cells. Bone marrow cells cultured in mesenchymal media were negative for p75, nestin and SOX-10, and did not express JCV T-antigen (C, F, I, L). Cell nuclei were visualized with DAPI. (Scale bar: 100 µm). (M) Diagram illustrating expression of JCV T-antigen in bone marrow cells. T-antigen expression was associated with neural crest lineage cells of the bone marrow cultured in serum-free neural stem cell media supplemented with bFGF and EFG (left panel), while mesenchymal cells maintained in alpha -MEM in the presence of 20% serum were negative for T-antigen (right panel). (N) FACS analysis of neural crest cells and MSCs demonstrates positivity for JCV T-antigen in 99% of neural crest cells and lack of JCV T-antigen expression in MSCs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23805194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Rat SOX10 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma tissue

Reviewed Applications

Read 1 review rated 5 using MAB2864 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References

  1. Lo, L. et al. (2002) Development 129:1553.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Entrez Gene IDs

6663 (Human); 20665 (Mouse); 29361 (Rat)

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for Human/Rat SOX10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat SOX10 Antibody

For research use only

Citations for Human/Rat SOX10 Antibody

Customer Reviews for Human/Rat SOX10 Antibody (1)

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  • Human/Rat SOX10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human melanoma
    Species: Human
    Verified Customer | Posted 12/05/2022
    Human melanoma with SOX10 mAB
    Human/Rat SOX10 Antibody MAB2864

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Protocols

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