Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 674801
View all RLK/TXK Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human RLK/TXK
Trp150-Val246
Accession # P42681

Specificity

Detects human RLK/TXK in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human RLK/TXK Antibody

Detection of Human RLK/TXK antibody by Western Blot.

Detection of Human RLK/TXK by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells. PVDF Membrane was probed with 1 µg/mL of Human RLK/TXK Monoclonal Antibody (Catalog # MAB6520) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RLK/TXK at approximately 58 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RLK/TXK Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RLK/TXK

RLK (resting lymphocyte kinase), also called TXK (T cell-expressed kinase) or PTK4, is a protein tyrosine kinase of the TEK family. It is expressed in T cells and some myeloid cells, especially in Th1 cells in inflammatory diseases such as rheumatoid arthritis and Behcet’s disease. Upon TCR stimulation, it translocates to the nucleus and induces IFN-gamma production. An alternate start site creates a resident nuclear 55 kDa form in addition to the larger (~58 kDa) form that is cytoplasmic or targeted to the plasma membrane via palmitoylation of a cysteine string motif. The region used as an immunogen is common to both forms and shares 79% and 81% amino acid identity with mouse and rat RLK, respectively.

 

Long Name

Resting Lymphocyte Kinase

Alternate Names

BTKL, PSCTK5, PTK4, TXK

Entrez Gene IDs

7294 (Human)

Gene Symbol

TXK

UniProt

P42681

Additional RLK/TXK Products

Product Documents for Human RLK/TXK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RLK/TXK Antibody

For research use only

Related Research Areas

Intracellular Kinases

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