Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 474315
View all S100A9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A9
Met1-Pro114
Accession # P06702

Specificity

Detects human S100A9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) S100A1, 2, 7, 8, 11, 13, rhS100B, rhS100P, recombinant mouse S100A4, 6, 10, or 16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S100A9 Antibody

Detection of Human S100A9 antibody by Western Blot.

Detection of Human S100A9 by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A9 antibody in Capan-1 Human Cell Line by Immunocytochemistry (ICC).

S100A9 in Capan-1 Human Cell Line.

S100A9 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human S100A9 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Capan-1 human pancreatic adenocarcinoma cell line

Western Blot

0.5 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A9

Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14 kDa member of the S100 family of EF-hand calcium-binding proteins. It is 114 amino acids (aa) in length and contains short sequential modules. There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions. S100A9 will noncovalently heterodimerize with S100A8. In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation. Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Entrez Gene IDs

6280 (Human); 20202 (Mouse)

Gene Symbol

S100A9

UniProt

P06702

Additional S100A9 Products

Product Documents for Human S100A9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A9 Antibody

For research use only

Citations for Human S100A9 Antibody

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Protocols

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