Human SALM2/LRFN1 Antibody

Catalog # Availability Size / Price Qty
MAB5669
MAB5669-SP
SALM2 in Human Cerebellum.
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Human SALM2/LRFN1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SALM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SALM1, 3, or 4 is observed.
Source
Monoclonal Mouse IgG2B Clone # 610712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SALM2
Gln32-Gly534 (predicted)
Accession # Q9P244
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry SALM2 in Human Cerebellum. View Larger

SALM2 in Human Cerebellum. SALM2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human SALM2/LRFN1 Monoclonal Antibody (Catalog # MAB5669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM2/LRFN1

SALM2 (synaptic adhesion-like molecule 2), also called LRFN1 (leucine-rich repeat and fibronectin type-III domain-containing protein 1) is a 105 kDa member of the LRFN family of type I transmembrane glycoproteins.  The 740 amino acid (aa) mature human SALM2 contains a 505 aa extracellular domain that includes seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain. Mature human SALM2 shares 99% aa identity with mouse and rat SALM2. SALM2 co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons and promotes neurite outgrowth.

Long Name
Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1
Entrez Gene IDs
57622 (Human); 365222 (Rat)
Alternate Names
KIAA1484; leucine rich repeat and fibronectin type III domain containing 1; leucine-rich repeat and fibronectin type III domain-containing protein 1; LRFN1; SALM2; synaptic adhesion-like molecule 2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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