Detection of Human SAMHD1 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SAMHD1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SAMHD1 by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for SAMHD1 at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human SAM (Sterile Alpha Motif) domain and HD (His/Asp motif-containing phosphohydrolase) domain-containing 1 (SAMHD1), also known as DCIP (dendritic cell IFN-g-induced protein) or MOP5 (monocyte protein 5) is a 70-80 kDa, 626 amino acid (aa) nuclear protein that acts as a dGTP-regulated deoxynucleotide triphosphohydrolase. It restricts HIV replication by binding nucleic acids. SAMHD-1 expression is induced by TNF-a in myeloid lineage cells and lung fibroblasts. Human SAMHD1 mutations are linked to Aicardi-Goutieres Syndrome, which is characterized by symptoms mimicking viral infection. Human SAMHD1 isoforms of 602, 591, and 556 aa lack aa 113-136, 502-536, and 285-354, respectively. The 591 and 556 aa forms are catalytically inactive. Within the region used as an immunogen, human SAMHD1 shares 85% aa sequence identity with mouse and rat SAMHD1.
SAM Domain and HD Domain 1
Entrez Gene IDs:
25939 (Human); 56045 (Mouse); 311580 (Rat)
AGS5; CHBL2; DCIP; DCIPMg11; Dendritic cell-derived IFNG-induced protein; EC 3.1.4.-; HDDC1; Monocyte protein 5; MOP5; MOP-5; MOP-5AGS5; SAM domain and HD domain 1; SAM domain and HD domain-containing protein 1; SAMHD1; SBBI88
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