Human SCF/c-kit Ligand Antibody

Catalog # Availability Size / Price Qty
MAB655-SP
MAB655-100
MAB655-500
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human SCF/c-kit Ligand Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human SCF in Western blots. In sandwich ELISAs, no significant cross-reactivity or interference was observed with 36 other cytokines.
Source
Monoclonal Mouse IgG1 Clone # 13302
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human SCF
Glu26-Ala189
Accession # P21583
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)

Human SCF/c-kit Ligand Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF255)

Standard: Recombinant Human SCF Protein (Catalog # 255-SC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

SCF, also known as c-kit ligand, mast cell growth factor (MGF) and steel-factor (SLF), is a pleiotropic cytokine that plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. SCF can exist either as a membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain.

Long Name
Stem Cell Factor
Entrez Gene IDs
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Product Datasheets

Citations for Human SCF/c-kit Ligand Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008;181(4):2772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007;109(10):4264-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Inhibition by glucocorticoids of the interleukin-1beta-enhanced expression of the mast cell growth factor SCF.
    Authors: Da Silva CA, 2019, Kassel O, Mathieu E, Massard G, Gasser B, Frossard N
    , 2002;135(7):1634-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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