Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13302
View all SCF/c-kit Ligand Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SCF
Glu26-Ala189
Accession # P21583

Specificity

Detects recombinant human SCF in Western blots. In sandwich ELISAs, no significant cross-reactivity or interference was observed with 36 other cytokines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human SCF/c‑kit Ligand Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)

Human SCF/c-kit Ligand Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF255)
  • Standard: Recombinant Human SCF Protein (Catalog # 255-SC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCF/c-kit Ligand

SCF, also known as c-kit ligand, mast cell growth factor (MGF) and steel-factor (SLF), is a pleiotropic cytokine that plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. SCF can exist either as a membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Gene Symbol

KITLG

UniProt

P21583

Additional SCF/c-kit Ligand Products

Product Documents for Human SCF/c‑kit Ligand Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SCF/c‑kit Ligand Antibody

For research use only

Citations for Human SCF/c‑kit Ligand Antibody

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