Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 333803
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Product Specifications

Immunogen

E. coli-derived recombinant human SCL/Tal1
Arg199-Gly266
Accession # P17542

Specificity

Detects human SCL/Tal1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human SCL/Tal1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human SCL/Tal1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCL/Tal1

SCL, also known as T-cell acute lymphocytic leukemia 1 (Tal1), is a basic helix-loop-helix transcription factor. Its expression is essential for hematopoietic lineage commitment of the hemangioblast.

Long Name

Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1

Alternate Names

Stem Cell Protein, Tal1, TCL5

Entrez Gene IDs

6886 (Human)

Gene Symbol

TAL1

UniProt

Additional SCL/Tal1 Products

Product Documents for Human SCL/Tal1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SCL/Tal1 Antibody

For research use only

Citations for Human SCL/Tal1 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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