Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 294301
View all SDNSF/MCFD2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SDNSF/MCFD2
Glu27-Gln146
Accession # Q8NI22

Specificity

Detects human SDNSF/MCFD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SDNSF is observed. In Western blots, no cross-reactivity with recombinant mouse SDNSF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human SDNSF/MCFD2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Recombinant Human SDNSF/MCFD2 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (Multiple Coagulation Factor Deficiency 2), was first identified as a secreted molecule that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of a complex that transports proteins from the ER to the Golgi.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Alternate Names

MCFD2

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Gene Symbol

MCFD2

UniProt

Q8NI22

Additional SDNSF/MCFD2 Products

Product Documents for Human SDNSF/MCFD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SDNSF/MCFD2 Antibody

For research use only

Related Research Areas

Coagulation

Citations for Human SDNSF/MCFD2 Antibody

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Protocols

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FAQs

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