Human Serpin B3/SCCA1 Antibody (886524)

R&D Systems | Catalog # MAB6528

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 886524
View all Serpin B3/SCCA1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B3/SCCA1
Asn2-Pro390
Accession # P29508

Specificity

Detects human Serpin B3/SCCA1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Serpin B3/SCCA1 Antibody (886524)

Detection of Human Serpin B3/SCCA1 antibody by Western Blot.

Detection of Human Serpin B3/SCCA1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B3/SCCA1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin B3/SCCA1 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

Serpin B3/SCCA1 in Human Squamous Cell Carcinoma.

Serpin B3/SCCA1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Serpin B3/SCCA1 Antibody (886524)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma

Western Blot

0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin B3/SCCA1

Serpin B3, also known as Squamous Cell Carcinoma Antigen-1 (SCCA1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).

References

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Benarafa, C. and Remold-O’Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
  3. Higgens, W.J. et al. (2010) J. Biol. Chem. 285:3722.
  4. Schick, C. et al. (1998) Biochemistry. 37:5258.

Alternate Names

SCCA1, T4-A

Entrez Gene IDs

6317 (Human)

Gene Symbol

SERPINB3

UniProt

P29508

Additional Serpin B3/SCCA1 Products

Product Documents for Human Serpin B3/SCCA1 Antibody (886524)

Certificate of Analysis

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Product Specific Notices for Human Serpin B3/SCCA1 Antibody (886524)

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Serpin B3/SCCA1 Antibody (886524)

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Protocols

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