Human Serpin B3/SCCA1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Serpin B3/SCCA1 by Western Blot.
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Human Serpin B3/SCCA1 Antibody Summary

Species Reactivity
Detects human Serpin B3/SCCA1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 886524
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B3/SCCA1
Accession # P29508
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
0.5 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Serpin B3/SCCA1 antibody by Western Blot. View Larger

Detection of Human Serpin B3/SCCA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B3/SCCA1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serpin B3/SCCA1 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

Serpin B3/SCCA1 in Human Squamous Cell Carcinoma. Serpin B3/SCCA1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B3/SCCA1

Serpin B3, also known as Squamous Cell Carcinoma Antigen-1 (SCCA1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Benarafa, C. and Remold-O’Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
  3. Higgens, W.J. et al. (2010) J. Biol. Chem. 285:3722.
  4. Schick, C. et al. (1998) Biochemistry. 37:5258.
Entrez Gene IDs
6317 (Human)
Alternate Names
HsT1196; Protein T4-A; SCC; SCCA; SCCA1; SCCA1SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; Squamous cell carcinoma antigen 1; T4-A

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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