Human Serpin B3/SCCA1 Antibody

Catalog # Availability Size / Price Qty
MAB6528
MAB6528-SP
Human Serpin B3/SCCA1 Antibody in Western Blot
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Serpin B3/SCCA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin B3/SCCA1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 886524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B3/SCCA1
Asn2-Pro390
Accession # P29508
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Serpin B3/SCCA1 by Western Blot. View Larger

Detection of Human Serpin B3/SCCA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B3/SCCA1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serpin B3/SCCA1 in Human Squamous Cell Carcinoma. View Larger

Serpin B3/SCCA1 in Human Squamous Cell Carcinoma. Serpin B3/SCCA1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B3/SCCA1

Serpin B3, also known as Squamous Cell Carcinoma Antigen-1 (SCCA1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).

References
  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Benarafa, C. and Remold-O’Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
  3. Higgens, W.J. et al. (2010) J. Biol. Chem. 285:3722.
  4. Schick, C. et al. (1998) Biochemistry. 37:5258.
Entrez Gene IDs
6317 (Human)
Alternate Names
HsT1196; Protein T4-A; SCC; SCCA; SCCA1; SCCA1SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; Squamous cell carcinoma antigen 1; T4-A

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Serpin B3/SCCA1 Antibody

There are currently no reviews for this product. Be the first to review Human Serpin B3/SCCA1 Antibody and earn rewards!

Have you used Human Serpin B3/SCCA1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review