Human Serum Amyloid A4 Antibody Summary
Accession # P35542
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Serum Amyloid A4 in Human Liver. Serum Amyloid A4 was detected in immersion fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human Serum Amyloid A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3856) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A4
Serum Amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis, and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.
Citation for Human Serum Amyloid A4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A proteomic approach identifies early pregnancy biomarkers for preeclampsia: novel linkages between a predisposition to preeclampsia and cardiovascular disease.
Authors: Blumenstein M, McMaster MT, Black MA, Wu S, Prakash R, Cooney J, McCowan LM, Cooper GJ, North RA
Sample Types: Plasma
Applications: Western Blot
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Cell and Tissue Staining Kits
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