Human Serum Amyloid A4 Antibody

Catalog # Availability Size / Price Qty
AF3856
AF3856-SP
Human Serum Amyloid A4 Antibody in Data
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Citations (1)
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Human Serum Amyloid A4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant mouse Serum Amyloid A4 is observed and less than 5% cross-reactivity with recombinant human (rh) Serum Amyloid A1 and rhSerum Amyloid A2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Serum Amyloid A4
Glu19-Tyr130
Accession # P35542
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serum Amyloid A4
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Serum Amyloid A4 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Serum Amyloid A4 in Human Liver. Serum Amyloid A4 was detected in immersion fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human Serum Amyloid A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3856) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A4

Serum Amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis, and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.

Entrez Gene IDs
6291 (Human); 20211 (Mouse); 365245 (Rat)
Alternate Names
C-SAA; C-SAACSAAConstitutively expressed serum amyloid A protein; SAA4; SAA5; serum amyloid A-4 protein; Serum Amyloid A4; serum amyloid A4, constitutive

Product Datasheets

Citation for Human Serum Amyloid A4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A proteomic approach identifies early pregnancy biomarkers for preeclampsia: novel linkages between a predisposition to preeclampsia and cardiovascular disease.
    Authors: Blumenstein M, McMaster MT, Black MA, Wu S, Prakash R, Cooney J, McCowan LM, Cooper GJ, North RA
    Proteomics, 2009;9(11):2929-45.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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