Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 667713
View all SMOC-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SMOC-2
Gln22-Gly446
Accession # Q9H3U7

Specificity

Detects human SMOC-2 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) SMOC-1 and no cross-reactivity with recombinant mouse SMOC-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SMOC‑2 Antibody

SMOC-2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

SMOC-2 in Human Ovarian Cancer Tissue.

SMOC-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human SMOC-2 Monoclonal Antibody (Catalog # MAB5140) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SMOC‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SMOC-2

SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins.  It is widely expressed, perhaps by endothelium, and apparently participates in both growth factor signaling and ECM organization.  Mature human SMOC-2 is 425 amino acids (aa) in length.  It contains one Kazal-like domain (aa 40-84), two thyroglobulin type-1 segments (aa 87-153 and 213-281), and two EF-hand sequences (aa 347-382 and 384‑419).  There are three splice variants.  One shows a 13 aa substitution for aa 442-446, another shows an 11 aa insertion after Thr170, and a third represents a combination of the above two variations.  Over aa 22-446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.

Long Name

Secreted Modular Calcium-binding Protein 2

Alternate Names

MST117, MSTP117, MSTP140, SMAP2, SMOC2

Entrez Gene IDs

64094 (Human); 64074 (Mouse); 292401 (Rat)

Gene Symbol

SMOC2

UniProt

Q9H3U7

Additional SMOC-2 Products

Product Documents for Human SMOC‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SMOC‑2 Antibody

For research use only

Citations for Human SMOC‑2 Antibody

Customer Reviews for Human SMOC‑2 Antibody

There are currently no reviews for this product. Be the first to review Human SMOC‑2 Antibody and earn rewards!

Have you used Human SMOC‑2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies