Human SODD/BAG4 Antibody

Catalog # Availability Size / Price Qty
AF4350
AF4350-SP

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Detection of Human SODD/BAG4 by Western Blot.
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Human SODD/BAG4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SODD/BAG4 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SODD/BAG4
Glu122-Leu457
Accession # O95429
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
SODD/BAG4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in SODD/BAG4 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human SODD/BAG4 by Western Blot. View Larger

Detection of Human SODD/BAG4 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for SODD/BAG4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry SODD/BAG4 in A431 Human Cell Line. View Larger

SODD/BAG4 in A431 Human Cell Line. SODD/BAG4 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human SODD/BAG4 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human SODD/BAG4 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SODD/BAG4 at approximately 72 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SODD/BAG4

Tumor necrosis factor receptor-1 (TNF-R1) and other TNF receptor superfamily members, such as DR3, contain intracellular death domains (DD) and are capable of initiating apoptosis when activated by their ligands. Silencer of Death Domains (SODD) was identified as being involved in the cellular mechanism to protect against ligand-independent signaling by TNF-R1 and other DD receptors. SODD, also known as Bcl-2-Associated Athanogene 4 (BAG4), is a 457 amino acid (aa), anti‑apoptotic protein that functions through interactions with a variety of proteins including Bcl-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kDa family. SODD is a ubiquitously expressed, cytoplasmic protein that contains a C‑terminal BAG domain that can bind and inhibit the chaperone activity of Hsc70/Hsp70. The association of SODD with the DD of TNF-R1 prevents constitutive activation of the TNF-R1 signaling pathway. Binding of TNF to TNF-R1 releases SODD and permits adapter molecules such as TRADD to associate with TNF-R1 leading to the activation of TNF signaling pathways such as apoptosis and NF kappa B activation.

Long Name
Silencer of Death Domains
Entrez Gene IDs
9530 (Human); 67384 (Mouse); 361167 (Rat)
Alternate Names
BAG4; BAG-4; BAG-family molecular chaperone regulator-4; Bcl-2-associated athanogene 4; BCL2-associated athanogene 4; Silencer of death domains; SODD; SODDBAG family molecular chaperone regulator 4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human SODD/BAG4 Antibody
By Ed Lo on 11/06/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human