Human SOX11 Antibody

SOX11 in Human Mantle Cell Lymphoma via seqIF™ staining on COMET™ SOX11 was detected in immersion fixed paraffin-embedded sections of human Mantle Cell Lymphoma using Mouse Anti-Human SOX11, Monoclonal Antibody (Catalog # MAB11734) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).​ Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of SOX11 in Human Glioblastoma. SOX11 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of SOX11 in human Mantle Cell Lymphoma SOX11 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human SOX11 by Western Blot. Western Blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX11 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.