Human SOX11 (SRY box-related gene 11) is a 60-72 kDa (46.6 kDa predicted), group C member of the SOX family of transcription factors. It is 441 amino acids (aa) in length, and contains one HMG box (aa 49-117), two poly-Gly regions (aa 144-214), one poly-Asp domain (aa 223-233) and one poly-Ser region. The HMG box determines DNA and transcriptional coactivator binding, the poly-Ser region lies in a potent transactivating region, and the poly-Asp domain autoinhibits/regulates activities of the other two regions. SOX11 appears early in development in multiple tissues, and impacts neuron, oligodendrocyte and cartilage formation. Human SOX11 is 94% aa identical to mouse SOX11 over aa 1-173. Mouse SOX11, however, is absent a 12 aa poly-Gly sequence that is present in human SOX11 between aa 145-156. Additional band correspondent to the lower molecular weight may represent SOX11 without posttranslational modifications or human SOX4, over aa 38-123 human SOX11 shares 100% aa identity with human SOX4. (1)
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, COMET
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1106960
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Product Specifications
Immunogen
E. coli - derived recombinant human SOX-11
Met1-Pro173
Accession # P35716
Met1-Pro173
Accession # P35716
Specificity
Detects recombinant human SOX11 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SOX11 Antibody
SOX11 in Human Mantle Cell Lymphoma via seqIF™ staining on COMET™
SOX11 was detected in immersion fixed paraffin-embedded sections of human Mantle Cell Lymphoma using Mouse Anti-Human SOX11, Monoclonal Antibody (Catalog # MAB11734) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Detection of SOX11 in Human Glioblastoma.
SOX11 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of SOX11 in human Mantle Cell Lymphoma
SOX11 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human SOX11 by Western Blot.
Western Blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX11 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human SOX11 Antibody
Application
Recommended Usage
COMET
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human glioblastoma and mantle cell lymphoma
Sample: Immersion fixed paraffin-embedded sections of human glioblastoma and mantle cell lymphoma
Western Blot
2 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line
Sample: SH-SY5Y human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SOX11
References
- Dictor, M. et al. (2009) Haematologica 94:1563.
Long Name
Transcription Factor SRY (Sex Determining Region Y)-box 11
Alternate Names
SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11
Gene Symbol
SOX11
UniProt
Additional SOX11 Products
Product Documents for Human SOX11 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SOX11 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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