Human STAT5a Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT5a. This antibody does not cross-react with STAT5b.
  • Source
    Monoclonal Mouse IgG1 Clone # 251610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human STAT5a synthetic peptide
    SLDSRLSPPAGLFTSARGSLS
    Accession # NP_003143
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed human peripheral blood mononuclear cells
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STAT5a in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT5a Monoclonal Antibody (Catalog # MAB21741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT5a

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

  • Long Name:
    Signal Transducer and Activator of Transcription 5
  • Entrez Gene IDs:
    6776 (Human); 20850 (Mouse)
  • Alternate Names:
    MGF; signal transducer and activator of transcription 5A; STAT5
Related Research Areas
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 93
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Mouse IgG1 Isotype Control

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Secondary Antibodies
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 5
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Goat Anti-Mouse IgG Antibody

WB AF007 3
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Goat Anti-Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002
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Goat Anti-Mouse IgG Fc Antibody

G-202-C 1
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001
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Donkey Anti-Mouse IgG (H+L) Antibody

D-201-C-ABS2
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