Human STAT5a Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT5a. This antibody does not cross-react with STAT5b.
  • Source
    Monoclonal Mouse IgG1 Clone # 251610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human STAT5a synthetic peptide
    SLDSRLSPPAGLFTSARGSLS
    Accession # NP_003143
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STAT5a in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT5a Monoclonal Antibody (Catalog # MAB21741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Immunocytochemistry
STAT5a in K562 Human Cell Line. STAT5a was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human STAT5a Monoclonal Antibody (Catalog # MAB21741) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT5a

STAT5a (Signal Transducer and Activator of Transcription-5a) is one of two closely related genes that belong to the STAT family of transcription factors. It is a 91 kDa cytosolic protein that contains an N-terminal domain (with an NES/Nuclear Export Signal) a coiled-coiled region (with an NLS/Nuclear Localization Signal) a DNA-binding site that recognizes a GAS (Gamma-interferon Activated Site) motif, an SH2 domain that allows for dimerization, and a C-terminal transactivation domain. STAT5a likely exists in a quiescent state as a cytoplasmic anti-parallel homodimer. Following activation of both tyrosine and non-tyrosine kinase membrane-bound receptors, STAT5a is phosphorylated, increasing its MW by some 5-6 kDa. Phosphorylated STAT5a will enter the nucleus as either a homodimer (or heterodimer with STAT5b), or as a complex with the intracellular domain of a tyrosine kinase receptor such as ErbB4. Once in the nucleus, STAT5a will form a homotetramer and bind either GAS or GAS-related sequences in gene regulatory regions. Notably, it is suggested that STAT5a may "cycle" through the nucleus without phosphorylation, a process that would seem to involve Importins alpha 3 and beta 1. STAT5a is related to STAT5b through gene duplication. They show 92% amino acid (aa) sequence identity, with the major differences existing over aa 45-56 and 773-794. The genes are not entirely redundant; STAT5a activates NDGR1 and SH2, while STAT5b regulates the Treg T cell genes, FoxP3 and CD25/IL-2R alpha.

  • Long Name:
    Signal Transducer and Activator of Transcription 5
  • Entrez Gene IDs:
    6776 (Human); 20850 (Mouse)
  • Alternate Names:
    MGF; signal transducer and activator of transcription 5A; STAT5
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 95  
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 14  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Goat Anti-Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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