Human SUSD2 Antibody

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Detection of Human SUSD2 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human SUSD2 Antibody Summary

Species Reactivity
Detects human SUSD2 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 944812
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK293-derived human SUSD2
Accession # Q9UGT4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SUSD2 antibody by Western Blot. View Larger

Detection of Human SUSD2 by Western Blot. Western blot shows lysates of SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB9056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SUSD2 at approximately 60 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SUSD2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

SUSD2 in Human Breast Cancer Tissue. SUSD2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB9056) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm with higher expression in cancer tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is  evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name
Sushi Domain Containing 2
Entrez Gene IDs
56241 (Human); 71733 (Mouse); 294335 (Rat)
Alternate Names
BK65A6.2; FLJ22778; SUSD2; Sushi domain (SCR repeat) containing; sushi domain containing 2; sushi domain-containing protein 2

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Citation for Human SUSD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SUSD2 expression correlates with decreased metastasis and increased survival in a high-grade serous ovarian cancer xenograft murine model
    Authors: JN Sheets, ME Patrick, KA Egland
    Oncotarget, 2020-06-16;11(24):2290-2301.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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