Human Syndecan-4 Antibody Summary
Accession # P31431
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Syndecan‑4 in HeLa Human Cervical Epithelial Carcinoma Cell Line. Syndecan-4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Syndecan-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Syndecan-4, previously known as amphiglycan or ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).
- Tkachenko, E. et al. (2005) Circ. Res. 96:488.
- Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
- David, G. et al. (1992) J. Cell Biol. 118:961.
- Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
- Charnaux, N. et al. (2005) FEBS J. 272:1937.
- Schmidt, A. et al., J. Biol. Chem. 280:34441.
- Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
- Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
- Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
- Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.
Citations for Human Syndecan-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
The Journal of Biological Chemistry, 2022;0(0):102029.
Sample Types: Cell Lysates
Contribution of syndecans to cellular uptake and fibrillation of alpha-synuclein and tau
Authors: A Hudák, E Kusz, I Domonkos, K Jósvay, AT Kodamullil, L Szilák, M Hofmann-Ap, T Letoha
Sci Rep, 2019;9(1):16543.
Sample Types: Cell Culture Supernates
Xentry, a new class of cell-penetrating peptide uniquely equipped for delivery of drugs.
Authors: Montrose K, Yang Y, Sun X, Wiles S, Krissansen G
Sci Rep, 2013;3(0):1661.
Sample Types: Whole Cells
Beta3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells.
Mol. Cancer, 2012;11(0):36.
Sample Types: Cell Lysates
Applications: Western Blot
Syndecan-1 and -4 differentially regulate oncogenic K-ras dependent cell invasion into collagen through alpha2beta1 integrin and MT1-MMP.
Authors: Vuoriluoto K, HÃƒÂ¶gnÃƒÂ¤s G, Meller P, Lehti K, Ivaska J
Matrix Biol., 2011;30(3):207-17.
Sample Types: Whole Cells
If Human Syndecan-4 is found on the membrane surface, and the immunogen is the extracellular portion of the protein, why does the ICC example for Catalog # AF2918 appear to have cytoplasmic staining?
Our ICC protocol includes a permeabilization step prior to staining with the antibody, so the antibody will stain Syndecan-4 if present in the cytoplasm in addition to Syndecan-4 on the cell surface.
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