Human Syndecan-4 Antibody AF2918: R&D Systems

Human Syndecan-4 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Syndecan-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Syndecan-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
    Glu19-Glu145
    Accession # P31431
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Syndecan‑4 (Catalog # 2918-SD)
  • Flow Cytometry
    0.25 µg/106 cells
    HeLa human cervical epithelial carcinoma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Syndecan‑4 in HeLa Human Cervical Epithelial Carcinoma Cell Line.
Syndecan‑4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Syndecan‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syndecan-4

Syndecan-4, previously known as amphiglycan or ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

  • References:
    1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
    2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
    3. David, G. et al. (1992) J. Cell Biol. 118:961. 
    4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
    5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
    6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
    7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
    8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
    9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
    10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.
  • Entrez Gene IDs:
    6385 (Human)
  • Alternate Names:
    Amphiglycan; MGC22217; Ryudocan core protein; Ryudocan; SDC4; SYND4ryudocan amphiglycan; syndecan 4 (amphiglycan, ryudocan); syndecan 4; syndecan proteoglycan 4; Syndecan4; Syndecan-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Xentry, a new class of cell-penetrating peptide uniquely equipped for delivery of drugs.
    Authors: Montrose K, Yang Y, Sun X, Wiles S, Krissansen G
    Sci Rep, 2013;3(0):1661.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  2. Beta3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells.
    Mol. Cancer, 2012;11(0):36.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Syndecan-1 and -4 differentially regulate oncogenic K-ras dependent cell invasion into collagen through alpha2beta1 integrin and MT1-MMP.
    Authors: Vuoriluoto K, Högnäs G, Meller P, Lehti K, Ivaska J
    Matrix Biol., 2011;30(3):207-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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