Human TACE/ADAM17 Cytosolic Domain Antibody

R&D Systems | Catalog # AF2129

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TACE
Asp695-Cys824
Accession # P78536

Specificity

Detects human TACE in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TACE/ADAM17 Cytosolic Domain Antibody

Detection of Human TACE/ADAM17 antibody by Western Blot.

Detection of Human TACE/ADAM17 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TACE/ADAM17 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

TACE/ADAM17 in Human Kidney Cancer Tissue.

TACE/ADAM17 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human TACE/ADAM17 antibody by Simple WesternTM.

Detection of Human TACE/ADAM17 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line, loaded at 0.2 mg/mL. A specific band was detected for TACE/ADAM17 at approximately 180 kDa (as indicated) in the HeLa parental cell line using 10 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human TACE/ADAM17 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human TACE/ADAM17 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using 1.

Applications for Human TACE/ADAM17 Cytosolic Domain Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TACE/ADAM17 (Catalog # 930-ADB), see our available Western blot detection antibodies

Knockout Validated

TACE/ADAM17 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in TACE/ADAM17 knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line 

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for Human TACE/ADAM17 Cytosolic Domain Antibody

Certificate of Analysis

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Product Specific Notices for Human TACE/ADAM17 Cytosolic Domain Antibody

For research use only

Citations for Human TACE/ADAM17 Cytosolic Domain Antibody

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Protocols

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