Human TBX18 Antibody (635305)

R&D Systems | Catalog # MAB63371

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 635305
View all TBX18 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX18
Ala384-Ser504
Accession # O95935

Specificity

Detects human TBX18 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX2, 3, 5, 6, 20, or rhT-bet is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TBX18 Antibody (635305)

TBX18 antibody in Saos-2 Human Cell Line by Immunocytochemistry (ICC).

TBX18 in Saos‑2 Human Cell Line.

TBX18 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TBX18 in A549 Human Cell Line.

TBX18 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF‑7 human breast cancer cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TBX18 Antibody (635305)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Saos‑2 human osteosarcoma cell line and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX18

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

Long Name

T-box Transcription Factor 18

Alternate Names

T-box 18, T-box protein 18, T-box transcription factor TBX18

Entrez Gene IDs

9096 (Human); 76365 (Mouse); 315870 (Rat)

Gene Symbol

TBX18

UniProt

O95935 (Human)

Additional TBX18 Products

Product Documents for Human TBX18 Antibody (635305)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX18 Antibody (635305)

For research use only

Related Research Areas

Cardiac Stem Cell Markers
Somite Markers

Citations for Human TBX18 Antibody (635305)

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