Human TGF-alpha Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TGF‑ alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) AR, rhBTC, rhEGF, rhHB-EGF, rhHRG-alpha, and rhHRG-beta is observed. Neutralizes the biological activity of rhTGF-alpha, but will not neutralize the biological activity of rhEGF.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    E. coli-derived recombinant human TGF-alpha
    Val40-Ala89
    Accession # P01135
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TGF-alpha (Catalog # 239-A)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human astrocytoma, pituitary adenoma, and prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
  • Neutralization
    Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.4-0.8  µg/mL in the presence of 3 ng/mL Recombinant Human TGF‑ alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by TGF‑ alpha and Neutralization by Human TGF‑ alpha  Antibody. Recombinant Human TGF‑ alpha (Catalog # 239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF‑ alpha (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF‑ alpha Polyclonal Antibody (Catalog # AB-239-NA). The ND50 is typically 0.4‑0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-alpha

TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.

  • Long Name:
    Transforming Growth Factor alpha
  • Entrez Gene IDs:
    7039 (Human)
  • Alternate Names:
    protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha
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