Human TIMP‑2 Antibody

R&D Systems | Catalog # MAB97112

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Goat IgG Clone # 40019A
View all TIMP-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line, CHO derived recombinant human TIMP-2
Cys27-Pro220
Accession # P16035

Specificity

Detects human TIMP-2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TIMP‑2 Antibody

Detection of Human TIMP-2 antibody by Western Blot.

Detection of Human TIMP‑2 by Western Blot.

Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB97112) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TIMP‑2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB97112) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
TIMP-2 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P).

TIMP‑2 in Human Pancreatic Cancer Tissue.

TIMP-2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB97112) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human TIMP‑2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human TIMP-2 knockout HeLa human cervical epithelial carcinoma cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB97112) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human TIMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Knockout Validated

TIMP‑2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in TIMP‑2 knockout HeLa human cervical epithelial carcinoma cell line.

Western Blot

1 µg/mL
Sample: Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023), HeLa human cervical epithelial carcinoma cell line, human placenta tissue, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Entrez Gene IDs

7077 (Human); 21858 (Mouse); 29543 (Rat)

Gene Symbol

TIMP2

UniProt

P16035

Additional TIMP-2 Products

Product Documents for Human TIMP‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TIMP‑2 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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Protocols

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