Human TLE2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TLE2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TLE1, 3, or 4 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 737428
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TLE2
    Leu11-Ser193
    Accession # Q04725
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TLE2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TLE2 Monoclonal Antibody (Catalog # MAB7216) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLE2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLE2

TLE2 (Transducin-Like Enhancer of Split 2; also ESG2 and Grg-2) is an 83-84 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is ubiquitously expressed and is known to antagonize TCF (Wnt)-mediated signaling. TLE2 binds to other family members as a heterooligomer or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of multiple factors such as FoxG1, Arx and histone H3. Human TLE2 is 743 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa 1-133), a CCN domain that contains an NLS (aa 195-256), and six WD repeats that mediate protein-protein interaction (aa 455-742). There are at least five potential phosphorylation sites that, if used, may account for SDS-PAGE MWs exceeding 95 kDa. Potential isoform variants exist. One possesses a 21 aa substitution for aa 1-9, while a second shows the same substitution coupled to an additional 10 aa substitution for aa 683-743. A third isoform possesses an alternative start site at Met56 coupled to a deletion of aa 124-190. Over aa 11-193, human TLE2 shares 91% aa identity with mouse TLE2.

  • Long Name:
    Transducin-like Enhancer of Split 2
  • Entrez Gene IDs:
    7089 (Human); 21886 (Mouse); 299636 (Rat)
  • Alternate Names:
    enhancer of split groucho 2; ESG; ESG2; ESG2Enhancer of split groucho-like protein 2; FLJ41188; GRG2; TLE2; transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 2, homolog of Drosophila E(sp1); transducin-like enhancer protein 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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