Human TMEM87A Antibody Summary
Accession # Q8NBN3
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TMEM87A by Simple WesternTM. Simple Western lane view shows lysate of T47D human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for TMEM87A at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of TMEM-87A in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of Human TMEM87A by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TMEM87A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TMEM87A (Transmembrane protein 87A) is predicted to be a 6-transmembrane, 555 amino acid (aa) glyco- and phospho-protein that is expressed on the cell surface. Its expression in the mammary gland is upregulated when there is a loss of caveolin-1 expression, which may confer susceptibility to breast cancer. Within the region used as an immunogen, human TMEM87A shares 83% aa sequence identity with mouse and rat TMEM87A. A potential isoform of 181 aa diverges C-terminal to aa 169, while another of 494 aa is divergent N-terminal to aa 70.
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