Human TNF RI/TNFRSF1A Antibody
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Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human TNF RI/TNFRSF1A Antibody Summary
Species Reactivity
Human
Specificity
Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 16803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Accession # NP_001056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
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Applications
Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.008-0.04 µg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Neutralization
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TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Flow Cytometry
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Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A
TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Product Datasheets
Citations for Human TNF RI/TNFRSF1A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
27
Citations: Showing 1 - 10
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TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
Authors: B Pesce, CH Ribeiro, M Larrondo, V Ramos, L Soto, D Catalán, JC Aguillón
International Journal of Molecular Sciences, 2022;23(16):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF
Authors: DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson
Nature Communications, 2021;12(1):583.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
PloS Pathogens, 2021;17(4):e1009522.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
Nat Commun, 2020;11(1):3459.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
Oncogene, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses
Authors: F Minshawi, MRH White, W Muller, N Humphreys, D Jackson, BJ Campbell, A Adamson, S Papoutsopo
Sci Rep, 2019;9(1):193.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes
Authors: M Monguió-To, M Franquesa, MR Sarrias, FE Borràs
Cell Death Dis, 2018;9(5):499.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
Authors: AT Holdbrooks, CM Britain, SL Bellis
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
PLoS ONE, 2017;12(8):e0183390.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers
Sci Rep, 2016;6(0):26321.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers
Sci Rep, 2016;6(0):26321.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
PLoS ONE, 2015;10(4):e0122212.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Enhancement of TWIK-related acid-sensitive potassium channel 3 (TASK3) two-pore domain potassium channel activity by tumor necrosis factor alpha.
Authors: El Hachmane M, Rees K, Veale E, Sumbayev V, Mathie A
J Biol Chem, 2014;289(3):1388-401.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
Authors: Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
Exp Cell Res, 2014;321(2):167-77.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
High efficiency cell-specific targeting of cytokine activity.
Authors: Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G
Nat Commun, 2014;5(0):3016.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts.
Authors: Mizrahi K, Yaniv I, Ash S, Stein J, Askenasy N
Bone Marrow Transplant, 2014;49(5):640-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
PLoS ONE, 2012;7(8):e42526.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
Authors: Kolliputi N, Waxman AB
Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Authors: Renaud SJ, Sullivan R, Graham CH
Placenta, 2009;30(4):313-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
J. Allergy Clin. Immunol., 2009;123(1):239-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
Exp. Hematol., 2008;36(12):1660-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma.
Authors: Dong HP, Kleinberg L, Silins I, Florenes VA, Trope CG, Risberg B, Nesland JM, Davidson B
Cancer, 2008;112(1):84-93.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
Cell Death Differ., 2006;13(10):1789-801.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Negative selection by apoptosis enriches progenitors in naive and expanded human umbilical cord blood grafts.
Authors: Mizrahi K, Ash S, Peled T, Yaniv I, Stein J, Askenasy N
Bone Marrow Transplant, 0;49(7):942-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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