TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.
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Human TNF RI/TNFRSF1A Antibody (16805)
R&D Systems | Catalog # MAB625
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Western Blot, Neutralization, Flow Cytometry, Antibody Array Development, Array Development, ELISA Capture, ELISA Development, ELISA Development (Capture), Functional Assay, Neutralizing
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 16805
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Product Specifications
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Extracellular domain
Extracellular domain
Specificity
Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TNF RI/TNFRSF1A Antibody (16805)
TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Detection of TNF RI/TNFRSF1A by Western Blot
Inhibition assays with antibody against tumor necrosis factor-receptor 1 (TNFR1AB). (A) Analysis of nuclear factor-kappa-B (NF-kappa B) phosphorylation upon blockade of TNFR1. Dental pulp stems (DPCs) were incubated with different concentrations (0, 4, 6, and 10 μg/mL) of TNFR1AB for 1 hour prior to treatment with TNF-alpha (10 ng/mL). Phosphorylation of NF-kappa B (p65, p105) was examined by Western blot at 5 minutes after stimulation. TNFR1AB inhibited TNF-alpha -induced phosphorylation of p105 and p65 in a dose-dependent manner, and a maximum effect was observed with a concentration of 10 μg/mL. (B) Flow cytometric analysis of expression of stage-specific embryonic antigen 4 (SSEA-4). TNFR1AB only partially inhibited TNF-alpha -induced increase in the number of SSEA-4+ cells. (C) Analysis of mRNA levels of OCT-4 and NANOG upon inhibition of TNFR1. Although TNF-alpha treatment increased the gene expression of OCT-4 and NANOG, TNFR1AB nullified the TNF-alpha -induced increase OCT-4 mRNA levels only. **P <0.01, one-way analysis of variance (ANOVA), Tukey test. Data are representative of at least three different experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24580841), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human TNF RI / TNFRSF1A ELISA Standard Curve
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) was serially diluted and captured by Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human TNF RI/TNFRSF1A Antibody (16805)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only
Sample: Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 0.3 μg/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Human TNF RI/TNFRSF1A Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB625 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TNF RI/TNFRSF1A
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A
Gene Symbol
TNFRSF1A
Additional TNF RI/TNFRSF1A Products
Product Documents for Human TNF RI/TNFRSF1A Antibody (16805)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TNF RI/TNFRSF1A Antibody (16805)
For research use only
Citations for Human TNF RI/TNFRSF1A Antibody (16805)
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Customer Reviews for Human TNF RI/TNFRSF1A Antibody (16805) (2)
5 out of 5
2 Customer Ratings
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Application: Block/NeutralizeSample Tested: Cell culture supernatantSpecies: HumanVerified Customer | Posted 12/15/2017
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/22/2017We used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
