Human TNF RI/TNFRSF1A Antibody MAB625-100: R&D Systems

Human TNF RI/TNFRSF1A Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 16805
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human TNF RI/TNFRSF1A
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
    under non-reducing conditions only
    • Human TNF RI/TNFRSF1A Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human TNF RI/TNFRSF1A Antibody (Catalog # MAB625)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF225)
  • ELISA Standard
     
    Recombinant Human sTNF RI/TNFRSF1A Protein (Catalog # 636-R1)
  • Neutralization
    Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 0.3 μg/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.

Recombinant Human TNF RI/TNFRSF1A (Catalog # 636‑R1) inhibits Recombinant Human TNF‑ alpha (Catalog # 210‑TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

 

  • Long Name:
    Tumor Necrosis Factor Receptor I
  • Entrez Gene IDs:
    7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
  • Alternate Names:
    CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016;6(0):34310.
    Species: Human
    Sample Type:
  2. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
    Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
    J Biol Chem, 2015;290(7):4022-37.
    Species: Human
    Sample Type: Complex Sample Type
    Application: ELISA Capture
  3. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012;5(0):702.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  5. IRF5 is required for late-phase TNF secretion by human dendritic cells.
    Authors: Krausgruber T, Saliba D, Ryzhakov G
    Blood, 2010;115(22):4421-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  7. Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
    Authors: Lee SY, Cherla RP, Tesh VL
    Infect. Immun., 2007;75(3):1291-302.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  8. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  9. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006;54(5):1451-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  11. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Array Development
  12. Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors.
    Authors: Hawari FI, Rouhani FN, Cui X, Yu ZX, Buckley C, Kaler M, Levine SJ
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(5):1297-302.
    Species: Human
    Sample Type: Cell Lysates
    Application: Flow
  13. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
    Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
    Exp. Cell Res., 2004;298(2):560-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death.
    Authors: Ricci-Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, Colombo A, Parati E, Peschle C, De Maria R
    J. Exp. Med., 2004;200(10):1257-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
    Authors: Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
    J. Immunol., 2002;169(10):5660-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
    Authors: Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
    Am. J. Gastroenterol., 2000;95(9):2338-42.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  17. Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
    Authors: Jyonouchi H, Sun S, Itokazu N
    Neuropsychobiology, 0;46(2):76-84.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 17 Citations
Isotype Controls
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Secondary Antibodies
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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 5 (Based on 1 review)

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ELISA Human TNF RI/TNFRSF1A Antibody MAB625
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 ELISA Human Anonymous 11/22/2017
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ELISA Human TNF RI/TNFRSF1A Antibody MAB625
ELISA: Human TNF RI/TNFRSF1A Antibody [MAB625]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWe used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.

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